Accessing Genetic Information with High-Density DNA Arrays
| Autor: | David Stern, Xiaohua C. Huang, Anthony Berno, Stephen P. A. Fodor, Earl Hubbell, Jim Winkler, Macdonald S. Morris, Robert Yang, Mark S. Chee, David J. Lockhart |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Mitochondrial DNA Gene Expression Computational biology Biology medicine.disease_cause DNA Mitochondrial Polymerase Chain Reaction Human mitochondrial genetics chemistry.chemical_compound Genetic linkage Molecular genetics medicine Humans Genetic variability Cloning Molecular Genetics Base Composition Mutation Genome Polymorphism Genetic Multidisciplinary Base Sequence Genetic Variation Nucleic Acid Hybridization Phycoerythrin Sequence Analysis DNA Fluoresceins Mitochondria chemistry Fluorescein Oligonucleotide Probes Molecular probe Algorithms DNA |
| Zdroj: | Science. 274:610-614 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | Rapid access to genetic information is central to the revolution taking place in molecular genetics. The simultaneous analysis of the entire human mitochondrial genome is described here. DNA arrays containing up to 135,000 probes complementary to the 16.6-kilobase human mitochondrial genome were generated by light-directed chemical synthesis. A two-color labeling scheme was developed that allows simultaneous comparison of a polymorphic target to a reference DNA or RNA. Complete hybridization patterns were revealed in a matter of minutes. Sequence polymorphisms were detected with single-base resolution and unprecedented efficiency. The methods described are generic and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|