Iodine staining outperforms phosphotungstic acid in high-resolution micro-CT scanning of post-natal mice cardiac structures
| Autor: | Leonard F Arnolda, A. Doost |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
business.industry
Soft tissue chemistry.chemical_element Iodine Stain Iodine staining 030218 nuclear medicine & medical imaging Staining Intensity (physics) 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry 030220 oncology & carcinogenesis Medicine Radiology Nuclear Medicine and imaging Tomography Phosphotungstic acid Ultrasonic Imaging and Tomography business Nuclear medicine |
| Zdroj: | J Med Imaging (Bellingham) |
| ISSN: | 2329-4302 |
| DOI: | 10.1117/1.jmi.8.2.027001 |
| Popis: | Purpose: Micro-computed tomography (micro-CT) scan provides high-resolution three-dimensional images of mineralized tissues in small animal models. Contrast enhancement is essential to visualize non-mineralized tissues with micro-CT scan. We attempted to compare the two most common contrast agents to stain and image mouse cardiac structures. Approach: Ex-vivo micro-CT scan images of the mouse hearts were obtained following staining by potassium iodide or phosphotungstic acid (PTA). PTA-stained samples were imaged after various durations following staining (14 days, 25 days, 187 days, and 780 days), whereas iodine-stained samples were imaged after 72 hours. We compared median staining intensity between PTA and iodine at 0.1-mm intervals from the edge using the Mann Whitney test with correction for multiple comparisons. Results: Sixty post-natal mice hearts were stained with either PTA or iodine and imaged using micro-CT scan. Iodine proved to be faster and more uniform in complete enhancement of cardiac tissue in as short as 72 h, whereas PTA required a significantly longer time period to penetrate mouse cardiac structure ([Formula: see text]). Median staining intensity with iodine was strongly higher than that with PTA from 0.1- to 1.5-mm distance from the epicardial edge (2-tailed [Formula: see text] value [Formula: see text] or lower throughout). Conclusions: Iodine-stained soft tissue imaging by micro-CT scan provides a non-destructive, efficient, and accurate visualization tool for anatomical analysis of animal heart models of human cardiovascular conditions. Iodine is more efficient compared to PTA to achieve complete murine myocardial staining in a significantly shorter time period. |
| Databáze: | OpenAIRE |
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