A simple ‘free 3H-ligand’ assay for rapid screening of hybridoma monoclonal antibodies against neurotransmitter analogs and anti-idiotype antibodies
| Autor: | D. Scott Linthicum, R.D. Simmons |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Ketanserin
medicine.drug_class Immunology Monoclonal antibody Serotonergic Mice chemistry.chemical_compound Immunoglobulin Idiotypes Piperidines medicine Animals Immunology and Allergy Receptor Neurotransmitter Hybridomas biology Chemistry Pimozide Antagonist Antibodies Monoclonal Ligand (biochemistry) Molecular biology Receptors Neurotransmitter Neurology biology.protein Haloperidol Neurology (clinical) Antibody medicine.drug |
| Zdroj: | Journal of Neuroimmunology. 11:245-249 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/0165-5728(86)90008-1 |
| Popis: | Effective, rapid screening of hybridoma supernatants for monoclonal antibodies against the dopaminergic antagonists pimozide and haloperidol, and the serotonergic antagonist ketanserin was performed using a 'free 3H-ligand' assay. Anti-mouse Ig-coated microtiter plates were incubated with hybridoma supernatants prior to incubation with excess 3H-ligand. After removal of free 3H-ligand, bound 3H-ligand was eluted with acid for liquid scintillation counting. With minor modification, the assay can be used to screen hybridomas for anti-anti-ligand (anti-idiotypic) antibodies. |
| Databáze: | OpenAIRE |
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