Dendritic cell-targeted porcine reproductive and respiratory syndrome virus (PRRSV) antigens adjuvanted with polyinosinic-polycytidylic acid (poly (I:C)) induced non-protective immune responses against heterologous type 2 PRRSV challenge in pigs
| Autor: | Drew R. Magstadt, Sakthivel Subramaniam, Pablo Piñeyro, Xiang-Jin Meng, Rachel J. Derscheid, Darin M. Madson |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Swine animal diseases medicine.medical_treatment Immunology Porcine Reproductive and Respiratory Syndrome Heterologous 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Immune system Antigen Adjuvants Immunologic medicine Animals Porcine respiratory and reproductive syndrome virus Antigens Viral General Veterinary biology Viral Vaccine Viral Vaccines 04 agricultural and veterinary sciences Dendritic Cells Porcine reproductive and respiratory syndrome virus biology.organism_classification Virology 030104 developmental biology Poly I-C chemistry Polyinosinic:polycytidylic acid biology.protein Antibody Adjuvant |
| Zdroj: | Veterinary immunology and immunopathology. 190 |
| ISSN: | 1873-2534 |
| Popis: | Porcine Reproductive and Respiratory Syndrome (PRRS) is an economically important swine viral disease worldwide. Current modified live-attenuated vaccines are ineffective against heterologous strains of PRRS virus (PRRSV) circulating in the field. In this study, we evaluated three dendritic cell (DC)-targeted vaccine candidates for their protective efficacy against heterologous PRRSV challenge. Ectodomain regions of DNA-shuffled structural proteins GP3, GP4, GP5 and M of PRRSV were fused together to form the vaccine antigen which was in turn fused with one of three recombinant antibodies each specific to a DC receptor: DC-SIGN, Langerin, and DEC205. The recombinant antibody-fused vaccine antigens were co-administered with polyinosinic-polycytidylic acid (poly (I:C)) adjuvant and subsequently challenged with a heterologous type 2 PRRSV strain (NADC20) in pigs. Our results demonstrate that pigs in DC-SIGN- and DEC205-targeted, but not Langerin- and non-targeted, vaccine groups showed significant IFN-γ- and IL-4-specific CD4T cell immune responses against the vaccine antigen in 7days post-challenge. Pigs in DC-SIGN- and Langerin-targeted vaccine groups showed greatly reduced IgG responses as compared to the DEC205- and non-targeted vaccine groups. The immune responses induced by DC-targeted vaccines did not reduce viremia and lung pathological lesions in type 2 PRRSV-challenged pigs. In contrast, pigs in Langerin-targeted vaccine group showed significantly increased serum viral titers and viral antigen in lung tissues at 7 and 14days post-challenge respectively. In conclusion, specific targeting of PRRSV antigen through DC-SIGN or DEC205 or Langerin-specific antibodies in the presence of poly (I:C) adjuvant induced immune responses that failed to protect pigs against heterologous type 2 PRRSV challenge. |
| Databáze: | OpenAIRE |
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