Dissecting Colistin Resistance Mechanisms in Extensively Drug-Resistant Acinetobacter baumannii Clinical Isolates
| Autor: | Marcel Tigges, Birgit Schellhorn, Marc Gitzinger, Sarah Gartenmann, Vincent Trebosc, Marcus Tötzl, Michel Pieren, Dirk Bumann, Christian Kemmer, Valentina Lucchini, Sergio Lociuro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Modern medicine
antibiotic resistance medicine.drug_class ethanolamine transferase Antibiotics Drug resistance mcr-1 Microbiology 03 medical and health sciences Antibiotic resistance Bacterial Proteins Virology Intensive care Drug Resistance Bacterial Gene Order medicine polycyclic compounds Humans colistin 030304 developmental biology epta 0303 health sciences biology 030306 microbiology Gene Expression Regulation Bacterial Therapeutics and Prevention biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses QR1-502 3. Good health Acinetobacter baumannii Anti-Bacterial Agents pmra Mutation Colistin bacteria MCR-1 acinetobacter baumannii medicine.drug Acinetobacter Infections Research Article |
| Zdroj: | mBio, Vol 10, Iss 4, p e01083-19 (2019) mBio mBio, Vol 10, Iss 4 (2019) |
| ISSN: | 2150-7511 |
| Popis: | The discovery of antibiotics revolutionized modern medicine and enabled us to cure previously deadly bacterial infections. However, a progressive increase in antibiotic resistance rates is a major and global threat for our health care system. Colistin represents one of our last-resort antibiotics that is still active against most Gram-negative bacterial pathogens, but increasing resistance is reported worldwide, in particular due to the plasmid-encoded protein MCR-1 present in pathogens such as Escherichia coli and Klebsiella pneumoniae. Here, we showed that colistin resistance in A. baumannii, a top-priority pathogen causing deadly nosocomial infections, is mediated through different avenues that result in increased activity of homologous phosphoethanolamine (PetN) transferases. Considering that MCR-1 is also a PetN transferase, our findings indicate that PetN transferases might be the Achilles heel of superbugs and that direct targeting of them may have the potential to preserve the activity of polymyxin antibiotics. Nosocomial infections with Acinetobacter baumannii are a global problem in intensive care units with high mortality rates. Increasing resistance to first- and second-line antibiotics has forced the use of colistin as last-resort treatment, and increasing development of colistin resistance in A. baumannii has been reported. We evaluated the transcriptional regulator PmrA as potential drug target to restore colistin efficacy in A. baumannii. Deletion of pmrA restored colistin susceptibility in 10 of the 12 extensively drug-resistant A. baumannii clinical isolates studied, indicating the importance of PmrA in the drug resistance phenotype. However, two strains remained highly resistant, indicating that PmrA-mediated overexpression of the phosphoethanolamine (PetN) transferase PmrC is not the exclusive colistin resistance mechanism in A. baumannii. A detailed genetic characterization revealed a new colistin resistance mechanism mediated by genetic integration of the insertion element ISAbaI upstream of the PmrC homolog EptA (93% identity), leading to its overexpression. We found that eptA was ubiquitously present in clinical strains belonging to the international clone 2, and ISAbaI integration upstream of eptA was required to mediate the colistin-resistant phenotype. In addition, we found a duplicated ISAbaI-eptA cassette in one isolate, indicating that this colistin resistance determinant may be embedded in a mobile genetic element. Our data disprove PmrA as a drug target for adjuvant therapy but highlight the importance of PetN transferase-mediated colistin resistance in clinical strains. We suggest that direct targeting of the homologous PetN transferases PmrC/EptA may have the potential to overcome colistin resistance in A. baumannii. |
| Databáze: | OpenAIRE |
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