Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model
| Autor: | Chih-Mei Cheng, Nai Wei Chen, Pin-Keng Shih, Hsien Pin Li, Chia Wei Chiu, Jian-Xun Chen, Shah Hwa Chou, Meei Feng Huang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Pulmonary Circulation Sildenafil Vasodilator Agents medicine.medical_treatment Ischemia Apoptosis Pharmacology Piperazines Sildenafil Citrate Rats Sprague-Dawley Random Allocation chemistry.chemical_compound medicine Animals Lung transplantation Sulfones Lung Saline bcl-2-Associated X Protein Bronchus Caspase 3 Interleukin-6 Tumor Necrosis Factor-alpha business.industry Left pulmonary artery medicine.disease Rats respiratory tract diseases Disease Models Animal medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 chemistry Purines Reperfusion Injury Anesthesia Surgery Tumor Suppressor Protein p53 business Reperfusion injury Lung Transplantation |
| Zdroj: | Journal of Surgical Research. 185:e77-e83 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2013.07.010 |
| Popis: | Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation. Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted to determine whether sildenafil could alleviate lung apoptosis and tissue injury in a rat model.Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline + I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and wet/dry (W/D) weight ratio.Compared with the saline + sham group, the saline + I/R group had significant increases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease in Bcl-2 (P0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight ratio but an increase in Bcl-2 expression (P0.05). Compared with the sildenafil + sham group, there were significant increases in p53 and TNF-α expression in the sildenafil + I/R group (P0.05).Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat model. |
| Databáze: | OpenAIRE |
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