Characterization of a Human Pancreatic Carcinoid in Vitro
| Autor: | Sun Kim, George H. Greeley, B. Haber, Jin Ishizuka, Srinivasan Rajaraman, R. D. Beauchamp, George Karp, Dilipkumar Parekh, Courtney M. Townsend, James C. Thompson |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Serotonin medicine.medical_specialty Endocrinology Diabetes and Metabolism Carcinoid tumors Mice Nude Receptors Cell Surface Carcinoid Tumor Biology Cytoplasmic Granules Pancreastatin Mice chemistry.chemical_compound Endocrinology Internal medicine Chromogranins Tumor Cells Cultured Internal Medicine medicine Animals Humans Autocrine signalling Neurotensin Hepatology Bombesin Chromogranin A Pancreatic Hormones medicine.disease Immunohistochemistry Molecular biology Pancreatic Neoplasms Microscopy Electron Somatostatin medicine.anatomical_structure chemistry biology.protein Secretagogue Pancreas Cell Division Neoplasm Transplantation |
| Zdroj: | Pancreas. 9:83-90 |
| ISSN: | 0885-3177 |
| Popis: | The study of functioning human endocrine tumors has been hampered by a lack of suitable in vitro models. We have established the first permanent cell line of a human pancreatic carcinoid tumor (BON) in culture. BON cells grow in monolayer culture and form colonies in soft agar. Injection of BON cells into nude mice produces transplantable tumors in a dose-dependent fashion. The histology of tumors in athymic mice from injection of dispersed, cultured BON cells is similar to the original histology of the resected tumor. Significant amounts of neurotensin, pancreastatin, and serotonin (5-HT) are demonstrated in the cells by radioimmunoassay (RIA) and the presence of chromogranin A, bombesin, and 5-HT is confirmed by immunocytochemistry. Numerous round and pleomorphic dense-core neurosecretory granules are present on electron microscopy. Functional receptors for acetylcholine, 5-HT, isoproterenol, and somatostatin are present on cultured cells. BON cells possess a specific transport system for uptake of 5-HT from the medium; this uptake system may be a route for regulation of autocrine effects of 5-HT on carcinoid cells. This unique human carcinoid tumor cell line should provide the opportunity for new insight into the biology of carcinoid tumors and of specific intracellular mechanisms for secretagogue action in the release of amines and peptides. |
| Databáze: | OpenAIRE |
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