Liberation of a fibrogenic factor from human blood monocytes, ascites cells, cultured histiocytes and transformed mouse macrophages by treatment with SiO2
| Autor: | Maija Aalto, Eino Kulonen, J Vilpo, C Sundström, Tapani Rönnemaa |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Proline Clinical Biochemistry Cell Biology Monocytes Cell Line Mice chemistry.chemical_compound Ribonucleases Fibrosis Synovial Fluid medicine Animals Ascitic Fluid Humans Cells Cultured Histiocyte Phagocytes Granuloma DNA synthesis Macrophages Fibrinogen Histiocytes DNA Neoplasms Experimental General Medicine Middle Aged Silicon Dioxide medicine.disease Molecular biology Culture Media Rats medicine.anatomical_structure chemistry Cell culture Liberation Thymidine |
| Zdroj: | Scandinavian Journal of Clinical and Laboratory Investigation. 40:311-318 |
| ISSN: | 1502-7686 0036-5513 |
| Popis: | Human monocytes and ascites macrophages from cirrhotic patients were isolated in Percoll-gradient and cultured with and without silica. Similar experiments were carried out also with cultured malignant human histiocytes and transformed mouse macrophages. The fibrogenic activity of the culture media was tested by measuring the incorporation of [3H]proline and [3H]thymidine into cultured rat granuloma and human synovial cells. Media from silica-treated monocytes, ascites macrophages and certain histiocyte and mouse macrophage lines caused an increase in the incorporation of both [3H]proline and [3H]thymidine into collagen and DNA, respectively, in both cell systems. Alkaline RNase activities were decreased markedly in the media from silica-treated ascites macrophages but not in the media of the monocytes or histiocytes. |
| Databáze: | OpenAIRE |
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