Rapid detection of Staphylococcus aureus Panton-Valentine leukocidin in clinical specimens by enzyme-linked immunosorbent assay and immunochromatographic tests
| Autor: | Mohamed Tazir, Eleanna Drougka, Cédric Badiou, Hélène Meugnier, Jerome Etienne, Iris Spiliopoulou, Graeme R. Nimmo, Oana Dumitrescu, Kristina G. Hulten, Narelle George, Andrea R. Forbes, Gerard Lina, Li Yang Hsu, Nadjia Ramdani-Bouguessa, Kian Sing Chan, Michèle Bes, François Vandenesch |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Staphylococcus aureus Micrococcaceae Virulence Factors Bacterial Toxins Exotoxins Biology medicine.disease_cause Staphylococcal infections Polymerase Chain Reaction Sensitivity and Specificity law.invention Microbiology Bacterial Proteins law Leukocidins medicine Humans Penicillin-Binding Proteins skin and connective tissue diseases Polymerase chain reaction Immunoassay Bacteriological Techniques Respiratory tract infections medicine.diagnostic_test SCCmec Bacteriology respiratory system biochemical phenomena metabolism and nutrition Staphylococcal Infections biology.organism_classification medicine.disease bacterial infections and mycoses Virology bacteria Panton–Valentine leukocidin |
| Zdroj: | Journal of clinical microbiology. 48(4) |
| ISSN: | 1098-660X |
| Popis: | Staphylococcus aureus strains producing Panton-Valentine leukocidin (PVL) have been epidemiologically linked to specific human infections. To evaluate immunological tests that may be used to diagnose infections with PVL-producing strains, we prospectively collected pus, respiratory tract specimens, and joint fluid specimens from which S. aureus had been isolated in clinical laboratories in six countries. An enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic test (ICT) targeting LukS-PV were performed directly with clinical samples for the detection of PVL. The same tests were applied to S. aureus culture supernatants. The corresponding S. aureus isolates were characterized by PCR for the presence of the PVL locus ( lukS-PV and lukF-PV ) and the mec A gene. A total of 185 samples from 144 skin infections, 23 bone and joint infections, and 18 lower respiratory tract infections were analyzed. By PCR, 72/185 S. aureus isolates were PVL locus positive (PVL + ); 28 of these were also mecA positive. PVL was detected in the supernatants of all PVL + strains by both ELISA and an ICT, while no signal was observed with PVL-negative strains. The PVL concentrations in human clinical samples that grew PVL + strains ranged from 0 to 399 μg/ml by ELISA. By the use of 0.015 μg/ml of PVL as a cutoff value, PVL was detected in 65/72 (90%) of the clinical samples by ELISA. The sensitivity and specificity of the ELISA test were 90% and 100%, respectively. By the ICT, PVL was detected in 57/72 (79%) of the samples, and the sensitivity and specificity of ICT were 79% and 100%, respectively. PVL is expressed by S. aureus during human infection, and a PVL-specific ELISA and ICT could be reliable tests for the diagnosis of infections caused by PVL-producing strains. |
| Databáze: | OpenAIRE |
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