FKBP5 gene expression in skeletal muscle as a potential biomarker for illegal glucocorticoid treatment in veal calves
| Autor: | Laura Starvaggi Cucuzza, Francesca Tiziana Cannizzo, Paola Pregel, Bartolomeo Biolatti |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
040301 veterinary sciences Prednisolone Gene Expression Biology Dexamethasone 0403 veterinary science Tacrolimus Binding Proteins 03 medical and health sciences chemistry.chemical_compound Glucocorticoid receptor Receptors Glucocorticoid Internal medicine medicine Animals Muscle Skeletal Glucocorticoids 030304 developmental biology 0303 health sciences Veal calves General Veterinary Estradiol Skeletal muscle 04 agricultural and veterinary sciences Biomarker FKBP medicine.anatomical_structure Endocrinology FKBP5 chemistry Gene Expression Regulation Biomarker (medicine) Cattle Hydroxysteroid hormones hormone substitutes and hormone antagonists Glucocorticoid Biomarkers Food Analysis medicine.drug |
| Zdroj: | Research in veterinary science. 133 |
| ISSN: | 1532-2661 |
| Popis: | For the current European legislation, the chemical analysis of drug residues is the exclusive accepted method to identify animals illicitly treated with growth promoters. Glucocorticoids and their metabolites are no detectable by LC/MS-MS methods in biological fluids when the growth promoter administration is discontinued several days prior to the slaughtering. The aim of this study was to elucidate the effect on the expression of genes belonging to the glucocorticoid pathway in three types of skeletal muscle of calves treated with prednisolone or dexamethasone in combination with estradiol. A gene expression change of glucocorticoid receptors (NR3C1 and NR3C2), their chaperones molecules (FKBP prolyl isomerase 4 and 5, FKBP4 and 5) and pre-receptor system (hydroxysteroid 11-beta dehydrogenases 1 and 2, HSD11B1 and 2) may indicate potential biomarkers of glucocorticoid treatment. In the biceps brachii muscle, the administration of dexamethasone with estradiol increased HSD11B2 (P 0.01) and NR3C2 (P 0.01) gene expression, whereas prednisolone administration increased HSD11B1 transcript levels (P 0.05). In the longissimus lumborum muscle, NR3C2 gene expression decreased following prednisolone administration (P 0.05). FKBP5 gene expression decreased in all considered muscles of calves administered with dexamethasone and estradiol (P 0.01), whereas increased in the longissimus lumborum (P 0.01) and vastus lateralis (P 0.05) muscle of prednisolone-treated group (P 0.05). The opposite effect of dexamethasone and prednisolone appears very promising to develop a low-cost screening test, because the expression analysis of a unique gene in a given tissue may distinguish the dispensed molecules. |
| Databáze: | OpenAIRE |
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