Transmissible spongiform encephalopathy in goats: is PrP rapid test sensitivity affected by genotype?
| Autor: | Simmons, Marion, Thorne, Leigh, Ortiz-Pelaez, Angel, Spiropoulos, John, Georgiadou, Soteria, Papasavva-Stylianou, Penelope, Andreoletti, Olivier, Hawkins, Stephen A.C., Hawkins, Stephen, Meloni, Daniela, Cassar, Claire |
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| Přispěvatelé: | Animal and Plant Health Agency [Addlestone, UK] (APHA), European Food Safety Authority (EFSA), Veterinary Services of Cyprus, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, This work was funded by the EC and Defra through the activities of the EURL for TSE at APHA. The UMR INRA ENVT 1225 was funded for this work by the EU FEDER/INTERREG (EFA148/16 REDPRION) |
| Rok vydání: | 2020 |
| Předmět: |
goats
Genotype Prions 040301 veterinary sciences [SDV]Life Sciences [q-bio] animal diseases Population Scrapie Disease Prion Proteins Epitope PRNP 0403 veterinary science 03 medical and health sciences medicine Animals Full Scientific Reports education transmissible spongiform encephalopathy 030304 developmental biology 0303 health sciences education.field_of_study Goat Diseases Polymorphism Genetic Transmissible spongiform encephalopathy General Veterinary biology scrapie 04 agricultural and veterinary sciences medicine.disease Virology nervous system diseases prion protein biology.protein ELISA Antibody |
| Zdroj: | Journal of Veterinary Diagnostic Investigation Journal of Veterinary Diagnostic Investigation, American Association of Veterinary Laboratory Diagnosticians, 2020, 32 (1), pp.87-93. ⟨10.1177/1040638719896327⟩ J Vet Diagn Invest |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | Transmissible spongiform encephalopathy (TSE) surveillance in goats relies on tests initially approved for cattle, subsequently assessed for sheep, and approval extrapolated for use in “small ruminants.” The current EU-approved immunodetection tests employ antibodies against various epitopes of the prion protein PrPSc, which is encoded by the host PRNP gene. The caprine PRNP gene is polymorphic, mostly at codons different from the ovine PRNP. The EU goat population is much more heterogeneous than the sheep population, with more PRNP-related polymorphisms, and with marked breed-related differences. The ability of the current tests to detect disease-specific PrPSc generated against these different genetic backgrounds is currently assumed, rather than proven. We examined whether common polymorphisms within the goat PRNP gene might have any adverse effect on the relative performance of EU-approved rapid tests. The sample panel comprised goats from the UK, Cyprus, France, and Italy, with either experimental or naturally acquired scrapie at both the preclinical and/or unknown and clinical stages of disease. Test sensitivity was significantly lower and more variable when compared using samples from animals that were preclinical or of unknown status. However, all of the rapid tests included in our study were able to correctly identify all samples from animals in the clinical stages of disease, apart from samples from animals polymorphic for serine or aspartic acid at codon 146, in which the performance of the Bio-Rad tests was profoundly affected. Our data show that some polymorphisms may adversely affect one test and not another, as well as underline the dangers of extrapolating from other species. |
| Databáze: | OpenAIRE |
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