Metabolomic tools to assess the chemistry and bioactivity of endophytic aspergillus strain
| Autor: | Grainne Abbott, Louise C. Young, Marc Diederich, RuAngelie Edrada-Ebel, Rothwelle J. Tate, Ahmed F. Tawfike, Christina Viegelmann, Marc Schumacher |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Cell Survival
Endophytic fungi Bioengineering Microbial Sensitivity Tests 01 natural sciences Biochemistry Endophyte Plant use of endophytic fungi in defense RS Metabolomics Cell Line Tumor Botany Humans Potential source HR-FT-MS Medicinal plants Molecular Biology Cell Proliferation Aspergillus Biological Products biology Bacteria Molecular Structure 010405 organic chemistry Plant Extracts Aspergillus aculeatus Terminalia General Chemistry General Medicine Dereplication biology.organism_classification Antineoplastic Agents Phytogenic NMR 0104 chemical sciences Anti-Bacterial Agents 010404 medicinal & biomolecular chemistry Molecular Medicine Drug Screening Assays Antitumor |
| ISSN: | 1612-1872 |
| Popis: | Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology that may find applications as pharmaceutical and agrochemical drugs. In this study, a combination of metabolomics and bioactivity-guided approaches were employed to isolate anticancer secondary metabolites from an endophytic Aspergillus aculeatus. The endophyte was isolated from the Egyptian medicinal plant Terminalia laxiflora and identified using molecular biological methods. Metabolomics and dereplication studies were accomplished by utilizing the MZmine software coupled with the universal Dictionary of Natural Products database. Metabolic profiling, with aid of multivariate data analysis, was performed at different stages of the growth curve to choose the optimised method suitable for up-scaling. The optimised culture method yielded a crude extract abundant with biologically-active secondary metabolites. Crude extracts were fractionated using different high-throughput chromatographic techniques. Purified compounds were identified by HRESI-MS, 1D and 2D-NMR. This study introduced a new method of dereplication utilising both high-resolution mass spectrometry and NMR spectroscopy. The metabolites were putatively identified by applying a chemotaxonomic filter. We also present a short review on the diverse chemistry of terrestrial endophytic strains of Aspergillus, which has become a part of our dereplication work and this will be of wide interest to those working in this field. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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