| Autor: |
Zhicheng Lu, Qi Li, Ke Xu, Jianming Pu, Jianguo Tian |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
AndrologiaREFERENCE. 53(11) |
| ISSN: |
1439-0272 |
| Popis: |
We mainly corroborated the potential mechanism of DLX1 and miR-129-5p in prostate cancer cells. DLX1 was upregulated in cancer cells according to qRT-PCR assay. We evaluated the functional changes of the transfected cells via Transwell assay, CCK-8 assay and wound healing assay. DLX1 was confirmed as a cancer promoter. In addition, qRT-PCR showed down-regulated miR-129-5p expression in prostate cancer. We further used dual-luciferase reporter detection to elucidate the targeting between these two genes. The inhibition of miR-129-5p on tumour was verified. Besides, co-transfection of oe-DLX1 and miR-129-5p mimics attenuated this inhibition. These data demonstrated functions of DLX1/miR-129-5p axis in prostate cancer: miR-129-5p hindered the biological functions of cancer cells via inhibiting DLX1 expression. We provide a novel biomarker for prostate cancer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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