Expression of Fas and Fas ligand protein and mRNA in mouse oocytes and embryos
| Autor: | R L Kelkar, Tarala D. Nandedkar, Shalmali J Dharma |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Embryology
animal structures Fas Ligand Protein Apoptosis Mice Inbred Strains In situ hybridization Biology Caspase 8 Fas ligand Embryonic and Fetal Development Mice Endocrinology medicine In Situ Nick-End Labeling Animals Blastocyst RNA Messenger fas Receptor In Situ Hybridization TUNEL assay Membrane Glycoproteins Embryogenesis Obstetrics and Gynecology Embryo Cell Biology Molecular biology Immunohistochemistry medicine.anatomical_structure Reproductive Medicine embryonic structures Oocytes Female Biomarkers |
| Zdroj: | Reproduction (Cambridge, England). 126(6) |
| ISSN: | 1470-1626 |
| Popis: | During mammalian embryonic development, abnormal eggs and embryos are eliminated by apoptosis; however, the precise apoptotic pathways remain as yet unidentified. In the present study, expression of Fas and Fas ligand - the proximal members of the death receptor pathway, was evaluated in mouse preimplantation embryos by immunofluorescence and in situ hybridization techniques. Ovulated oocytes were collected from oviducts of cyclic mice on the day of oestrus (day 0), and one-cell, two-cell embryos and eight-cell morulae were collected from oviducts of mated animals on days 1, 2 and 3 of pregnancy, respectively. Blastocysts were flushed from the uterine horns on day 4. Expression of Fas and Fas L mRNAs and proteins was absent from embryos at days 0, 1 and 2. A marked increase in Fas and Fas L mRNA and protein expression was detected in all morphologically normal embryos on day 3 and day 4. In addition, embryos on days 3 and 4 were positive for terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) staining; however, absence of caspase 8 and 3 and intense localization of proliferating cell nuclear antigen confirmed the proliferative status of these embryos. Furthermore, TUNEL staining was absent in postimplantation embryonic sections obtained on day 6. The results of the present studies thus indicate an equilibrium between proliferation and apoptosis in the preimplantation embryo. |
| Databáze: | OpenAIRE |
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