Changes in oxidative phosphorylation caused by ethyl -trichloropropionate
| Autor: | C.C. Kratzing, J.N. Mansbridge |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Biophysics
Stimulation Mitochondria Liver Oxidative phosphorylation Acetates Biochemistry Oxidative Phosphorylation Phosphates chemistry.chemical_compound Adenosine Triphosphate Oxygen Consumption Diamine Respiration Animals Molecular Biology Ethanol Chemistry Glutamate receptor Substrate (chemistry) Phosphorus Isotopes Phosphate Stimulation Chemical Rats Adenosine Diphosphate Enzyme Activation Butyrates Depression Chemical Dinitrophenol Chlorine Propionates Mitochondrial Swelling Drug Antagonism Dinitrophenols |
| Zdroj: | Archives of biochemistry and biophysics. 150(1) |
| ISSN: | 0003-9861 |
| Popis: | The effects of ethyl βββ-trichloropropionate on respiration, ATPase activity, and the ATP/phosphate exchange reactions of rat liver mitochondria have been investigated. The major effect of the drug on respiration is a marked inhibition of ADP- or dinitrophenol-stimulated respiration with glutamate plus malate, succinate, or ascorbate plus tetramethyl-p-phenylene diamine as substrate. The inhibition is most marked with glutamate plus malate. Ethyl βββ-trichloropropionate at low concentration (up to 0.8 mm) also causes a slight stimulation of respiration in the absence of ADP or DNP when glutamate plus malate or succinate is the substrate. At similar concentrations to those at which it inhibits respiration, ethyl βββ-trichloropropionate stimulated mitochondrial ATPase activity to a very large extent. The relation between ATPase activity and drug concentration was sigmoid and the greatest rate obtained was higher than the maximum rate obtained with dinitrophenol. Dinitrophenol was antagonistic to ethyl βββ-trichloropropionate. Ethyl βββ-trichloropropionate inhibited the ATP/phosphate exchange reaction. The activities of a number of chloroalkyl esters related to ethyl βββ-trichloropropionate are compared and the connection between the results in the cell-free system and the biological effects of the compound in the whole animal are discussed. |
| Databáze: | OpenAIRE |
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