Fructus Lycii and Salvia miltiorrhiza Bunge extract alleviate retinitis pigmentosa through Nrf2/HO-1 signaling pathway
| Autor: | Yi-Jing Yang, Zhen Yao, Jun Peng, Chen Ou, Jiang Pengfei, Qing-Hua Peng, Meiyan Zeng, Ye Tian, Houpan Song |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
NF-E2-Related Factor 2 Salvia miltiorrhiza Oxidative phosphorylation Pharmacology medicine.disease_cause Retina Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Mice Random Allocation 0302 clinical medicine Western blot Drug Discovery medicine Electroretinography Animals Outer nuclear layer 030304 developmental biology chemistry.chemical_classification 0303 health sciences medicine.diagnostic_test biology Plant Extracts Glutathione peroxidase Membrane Proteins Lycium Malondialdehyde Mice Inbred C57BL Oxidative Stress medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Fruit biology.protein Female Oxidative stress Biomarkers Heme Oxygenase-1 Retinitis Pigmentosa Drugs Chinese Herbal |
| Zdroj: | Journal of ethnopharmacology. 273 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Fructus Lycii and Salvia miltiorrhiza Bunge (FS) are popular Chinese herbs for the treatment of retinitis pigmentosa (RP). Aim of the study This study was to evaluate protective effects of FS extract on RP and to explore whether FS extract exerts its protective effects via oxidative stress by regulating Nrf2/HO-1 signaling pathway. Material and methods FS extract were identified by UPLC chromatographic analysis. Rd10 mice as the model of RP, followed by a 4-week FS extract treatment by intragastric administration. After the animal sacrifice, histopathological examination and Scotopic electroretinography (ERG) analysis were assessed. The oxidative stress markers were determined and the expression levels of Nrf2 and HO-1 mRNA were evaluated by qRT-PCR. The expression and distribution of Nrf2 and HO-1 protein were determined by Western blot and immunohistochemistry. Results The morphological changes of Outer nuclear layer (ONL) thickness and number of the ONL were observed with a significant increased, and the functional changes of a-amplitude and b-wave amplitude were measured with a markedly increased. Treatment with FS extract remarkably increased levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and decreased level of malondialdehyde (MDA). Moreover, FS extract up-regulated mRNA and protein expression of Nrf2 and HO-1. Conclusions This study indicated that FS extract can improve retinal morphology and function, which may have occurred through the regulation of the Nrf2/HO-1 pathway to inhibit the oxidative reaction. |
| Databáze: | OpenAIRE |
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