Rhubarb attenuates blood-brain barrier disruption via increased zonula occludens-1 expression in a rat model of intracerebral hemorrhage
Autor: | Fan Peng, Tao Tang, Jie‑Kun Luo, Gui Xie, Yang Wang, Haigang Li, Ze‑Qi Chen |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty Pharmacology Blood–brain barrier traditional Chinese medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immunology and Microbiology (miscellaneous) Medicine cardiovascular diseases Evans Blue Intracerebral hemorrhage Oncogene business.industry Articles rhubarb General Medicine blood-brain barrier medicine.disease intracerebral hemorrhage Molecular medicine Pathophysiology Extravasation zonula occludens-1 030104 developmental biology medicine.anatomical_structure chemistry Apoptosis business 030217 neurology & neurosurgery |
Zdroj: | Experimental and Therapeutic Medicine |
ISSN: | 1792-1015 1792-0981 |
Popis: | Blood-brain barrier (BBB) disruption is a key pathophysiological factor of intracerebral hemorrhage (ICH). The level of zonula occludens-1 (ZO-1) has been closely associated with the degree of BBB damage, and is an indicator of BBB destruction. The aim of the present study was to evaluate the effects of rhubarb on BBB function in a rat model of ICH. ICH was induced in rats by treatment with type VII collagenase. Sham-operated rats were administered with an equal volume of saline. Following the administration of rhubarb decoction (20 g/kg), neurobehavioral function evaluation and Evans blue extravasation assays were performed at days 1, 3 and 5 after ICH. ZO-1 expression in the brain of ICH-induced rats were analyzed via reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analyses. The results suggested that rhubarb significantly ameliorated neurological symptoms and attenuated BBB permeability. The results of immunohistochemistry and RT-PCR studies indicated that the expression of ZO-1 expression was robust in the sham-operated group and was weak in the vehicle-treated group at day 3. The present data indicated that rhubarb effectively attenuated ICH-induced BBB damage in rats, raising the possibility that rhubarb or its active components may be considered useful as neuroprotective drugs for ICH. The protective mechanisms appeared to involve the preservation of BBB integrity and elevation of ZO-1 protein expression levels. |
Databáze: | OpenAIRE |
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