Quantitive cytokine mRNA expression profiles in the colonic mucosa of patients with steroid naïve ulcerative colitis during active and quiescent disease
| Autor: | Chikako Tokoro, Ichiro Kawana, Satoshi Umemura, Shinichiro Suzuki, Tsuyoshi Yamamoto, Taiki Morohashi, Tomoko Koide, Daisuke Takahashi, Tenʼi Godai, Reikei Matsuda, Yuji Fujita |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology Cryptitis Biopsy Drug Resistance Administration Oral Gastroenterology Inflammatory bowel disease Polymerase Chain Reaction Intestinal mucosa Immunology and Allergy Intestinal Mucosa Mesalamine biology Anti-Inflammatory Agents Non-Steroidal Remission Induction Colonoscopy Middle Aged Prognosis Ulcerative colitis medicine.anatomical_structure Cytokines Female Adult medicine.medical_specialty Colon Proinflammatory cytokine Young Adult Internal medicine medicine Humans Leukapheresis RNA Messenger Colitis Interleukin 6 Retrospective Studies Lamina propria Dose-Response Relationship Drug business.industry Interleukin-6 Tumor Necrosis Factor-alpha Interleukin-8 medicine.disease Gene Expression Regulation biology.protein Colitis Ulcerative business Follow-Up Studies Granulocytes |
| Zdroj: | Inflammatory bowel diseases. 15(3) |
| ISSN: | 1536-4844 |
| Popis: | Background: Cytokines have validated roles in the immunopathogenesis of inflammatory bowel disease (IBD). This study was to investigate the expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, and IL-10 mRNAs in the colonic mucosa of patients with ulcerative colitis (UC) during active and quiescent UC. Methods: At colonoscopy, biopsies were taken from inflamed and non-inflamed mucosa of patients with steroid-naive UC (n = 15), non-IBD inflammatory colitis controls (ICC, n = 6), and non-colitis controls (NCC, n = 14). The presence of extensive mononuclear cells and neutrophils infiltrate in the lamina propria, cryptitis, and epithelial damage defined an inflammatory lesion in the mucosa. Quantitative cytokine mRNA expressions in biopsies were measured by real-time polymerase chain reaction (PCR). Results: Of 15 UC patients, 3 remitted with 5-aminosalicylate and 11 received granulocytapheresis; of these, 10 remitted. At baseline, IL-6, IL-8, TNF-α, and IL-10 mRNAs were high in inflamed mucosa compared with NCC (P < 0.01). In active UC, IL-6, IL-8 and IL-10 mRNAs were high compared with non-inflamed mucosa (P = 0.03, P = 0.03, P < 0.05, respectively). Both TNF-α mRNA (P = 0.03) and IL-6 mRNA (P = 0.04) were higher in UC compared with ICC. Even in non-inflamed mucosa, IL-8 and TNF-α mRNA expressions were high compared with NCC. Both IL-6 and IL-8 mRNAs decreased to normal levels after granulocytapheresis. Conclusions: During active UC, all 4 cytokine mRNA levels were high; only IL-6 and IL-8 mRNAs decreased to normal levels during remission. IL-8 mRNA was high even at sites of endoscopically quiescent UC during active disease. Steroid naive patients respond well to granulocytapheresis. (Inflamm Bowel Dis 2008) |
| Databáze: | OpenAIRE |
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