Spectral characterization of liquid hemoglobin phantoms with varying oxygenation states
| Autor: | Motasam Majedy, Marcus Larsson, Rolf B. Saager, Tomas Strömberg, E. Göran Salerud |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Paper
Materials science Absorption spectroscopy Atom and Molecular Physics and Optics tissue simulating phantom Medical Laboratory and Measurements Technologies Biomedical Engineering Analytical chemistry chemistry.chemical_element hemoglobin oxygen saturation Oxygen Imaging phantom Methemoglobin Collimated light Biomaterials Hemoglobins Spectroscopy Medicinsk laboratorie- och mätteknik Oxygen saturation (medicine) Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials chemistry Special Section on Tissue Phantoms to Advance Biomedical Optical Systems Oxygen Saturation Oxyhemoglobins Atom- och molekylfysik och optik Hemoglobin |
| Zdroj: | Journal of Biomedical Optics |
| ISSN: | 1560-2281 1083-3668 |
| Popis: | Significance: For optical methods to accurately assess hemoglobin oxygen saturation in vivo, an independently verifiable tissue-like standard is required for validation. For this purpose, we propose three hemoglobin preparations and evaluate methods to characterize them. Aim: To spectrally characterize three different hemoglobin preparations using multiple spectroscopic methods and to compare their absorption spectra to commonly used reference spectra. Approach: Absorption spectra of three hemoglobin preparations in solution were characterized using spectroscopic collimated transmission: whole blood, lysed blood, and ferrous-stabilized hemoglobin. Tissue-mimicking phantoms composed of Intralipid, and the hemoglobin solutions were characterized using spatial frequency-domain spectroscopy (SFDS) and enhanced perfusion and oxygen saturation (EPOS) techniques while using yeast to deplete oxygen. Results: All hemoglobin preparations exhibited similar absorption spectra when accounting for methemoglobin and scattering in their oxyhemoglobin and deoxyhemoglobin forms, respectively. However, systematic differences were observed in the fitting depending on the reference spectra used. For the tissue-mimicking phantoms, SFDS measurements at the surface of the phantom were affected by oxygen diffusion at the interface with air, associated with higher values than for the EPOS system. Conclusions: We show the validity of different blood phantoms and what considerations need to be addressed in each case to utilize them equivalently. Funding: This research was financially supported by VINNOVA grants via the Swelife and MedTech4Health programs (Grant Nos. 2016-02211, 2017-01435, and 2019-01522) and the Knut and Alice Wallenberg Foundation’s Center for Molecular Medicine at Linköping University (WCMM). |
| Databáze: | OpenAIRE |
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