A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration
| Autor: | Marzuk Ahmed, Zaid Bin Mahbub, Sharif Hasan, Salma Akter, Md. Kabir Ahamed, Tawfika Nasrin, Mohammad Abu Sayem Karal, Shareef Ahammed |
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| Rok vydání: | 2021 |
| Předmět: |
Polymers
Analytical chemistry 02 engineering and technology law.invention Suspension (chemistry) chemistry.chemical_compound law Materials Flow Rate 0303 health sciences Multidisciplinary Physics Vesicle Classical Mechanics Fluoresceins 021001 nanoscience & nanotechnology Volumetric flow rate Chemistry Separation Processes Membrane Macromolecules Cell Processes Physical Sciences Log-normal distribution Phosphatidylcholines Medicine Cellular Structures and Organelles Swelling medicine.symptom 0210 nano-technology Research Article Statistical Distributions Materials science Imaging Techniques Science Materials Science Fluid Mechanics Research and Analysis Methods Skewness Continuum Mechanics 03 medical and health sciences Fluorescence Imaging medicine Vesicles Polycarbonates Unilamellar Liposomes Filtration 030304 developmental biology Biology and Life Sciences Fluid Dynamics Cell Biology Polymer Chemistry Probability Theory Probability Distribution Cellular Extrusion Calcein chemistry Mathematics |
| Zdroj: | PLoS ONE, Vol 16, Iss 7, p e0254930 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | A new purification technique is developed for obtaining distribution of giant unilamellar vesicles (GUVs) within a specific range of sizes using dual filtration. The GUVs were prepared using well known natural swelling method. For filtration, different combinations of polycarbonate membranes were implemented in filter holders. In our experiment, the combinations of membranes were selected with corresponding pore sizes–(i) 12 and 10 μm, (ii) 12 and 8 μm, and (iii) 10 and 8 μm. By these filtration arrangements, obtained GUVs size distribution were in the ranges of 6−26 μm, 5–38 μm and 5–30 μm, respectively. In comparison, the size distribution range was much higher for single filtration technique, for example, 6−59 μm GUVs found for a membrane with 12 μm pores. Using this technique, the water-soluble fluorescent probe, calcein, can be removed from the suspension of GUVs successfully. The size distributions were analyzed with lognormal distribution. The skewness became smaller (narrow size distribution) when a dual filtration was used instead of single filtration. The mode of the size distribution obtained in dual filtration was also smaller to that of single filtration. By continuing this process of purification for a second time, the GUVs size distribution became even narrower. After using an extra filtration with dual filtration, two different size distributions of GUVs were obtained at a time. This experimental observation suggests that different size specific distributions of GUVs can be obtained easily, even if GUVs are prepared by different other methods. |
| Databáze: | OpenAIRE |
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