Diet quality and NSAIDs promote changes in formation of prostaglandins by an aquatic invertebrate

Autor: Albert Serra-Compte, Damià Barceló, Sara Rodríguez-Mozaz, Julio César López-Doval, Sergi Sabater
Rok vydání: 2020
Předmět:
musculoskeletal diseases
Ketoprofen
Naproxen
Diclofenac
Environmental Engineering
Health
Toxicology and Mutagenesis
0208 environmental biotechnology
Ibuprofen
02 engineering and technology
010501 environmental sciences
Pharmacology
01 natural sciences
chemistry.chemical_compound
medicine
Animals
Environmental Chemistry
skin and connective tissue diseases
0105 earth and related environmental sciences
Invertebrate
Arachidonic Acid
Anti-Inflammatory Agents
Non-Steroidal
Public Health
Environmental and Occupational Health
food and beverages
Lipid metabolism
General Medicine
General Chemistry
Metabolism
Lipid Metabolism
Invertebrates
Pollution
Diet
020801 environmental engineering
carbohydrates (lipids)
chemistry
Prostaglandins
lipids (amino acids
peptides
and proteins)
Arachidonic acid
medicine.drug
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2020.126892
Popis: We used the freshwater insect Hydropsyche sp. to investigate the impact of diets lacking arachidonic acid (ARA) and an environmentally relevant mixture of NSAIDs (Ibuprofen, Ketoprofen, Diclofenac and Naproxen at a nominal concentration of all compounds together 16.75 μg L) on their metabolism of ARA and prostaglandins (PGs). The organisms were exposed for 16 days to four different treatments: a reference (FF), a diet lacking ARA (O), to NSAIDs in water (FFN) and to the combination of the two factors (ON). Mortality, biomass and bioconcentration of pharmaceuticals were investigated. The ARA and PGs levels in the organisms were monitored by utilising a targeted metabolomics approach. NSAIDs or dietary constraints did not produce significant differences in biomass or mortality of Hydropsyche sp. among treatments. In organisms exposed to NSAIDs, all pharmaceuticals were detected, except for Ketoprofen. Metabolomic approach determined the presence of PGH, PGE and PGD. Levels of ARA diminished significantly in those organisms in treatment ON. The levels of PGs responded negatively to the absence of ARA in diet: PGH diminished significantly with respect to the reference in treatment O while PGE diminished significantly in treatment ON. Regarding the effects of NSAIDs on ARA metabolism, our results suggest that it was sensitive to NSAIDs, but effects were weak and did not imply a general decrease in the PGs. We confirmed that ARA was the main substrate for the synthesis of PGs in Hydropsyche sp, their absence or poor levels of ARA in diet, produced changes in the PG levels.
This work was supported by GLOBAQUA project (FP7, No 603629), the Spanish Ministry of Economy and Competitiveness through the projects CLIMALERT (PCIN-2017- 068) and SPACESTREAM (CGL 2017-88640-C2-1-R) and by the Generalitat de Catalunya (Consolidated Research Group “2014 SGR 291 – ICRA”). ICRA researchers thank funding from CERCA program. Julio C. López-Doval thanks the program Juan de la Cierva from the Spanish Ministry of Economy and Competitiveness (IJCI-2015-23644). Albert Serra-Compte benefits from an FI-DGR research fellowship from the Catalan Government (2016FI_B00601). Sara Rodriguez-Mozaz acknowledges the Ramon y Cajal program (RYC- 2014–16707) from the Spanish State Research Agency of Spanish Ministry of Science, Innovation and Universities (AEI-MCIU). Carmen Gutierrez Provecho and Juan David González-Trujillo (ICRA) are gratefully acknowledged for their assistance in the field collections and lab processing of the samples.
Databáze: OpenAIRE
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