BALB/C 3T3 CELLS CO-EXPRESSING FGF-2 AND SOLUBLE FGF RECEPTOR ACQUIRE TUMORIGENICITY
| Autor: | Hidenori Yamada, Masaharu Seno, Atsushi Nishimura, Megumi Kosaka, Reiko Sasada, Satimaru Seno, Hiroko Tada, Akinori Masago, Koichi Igarashi |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Carcinogenicity Tests
Immunology Gene Expression Mice Nude Biology Fibroblast growth factor Biochemistry 3T3 cells Extracellular matrix Mice In vivo Complementary DNA medicine Extracellular Animals Humans Immunology and Allergy Secretion Receptor Molecular Biology Mice Inbred BALB C 3T3 Cells Hematology Receptors Fibroblast Growth Factor Molecular biology Cell biology medicine.anatomical_structure Solubility Fibroblast Growth Factor 2 |
| Zdroj: | Cytokine. 10:290-294 |
| ISSN: | 1043-4666 |
| DOI: | 10.1006/cyto.1997.0286 |
| Popis: | The physiological significance of the soluble fibroblast growth factor (FGF) receptors is not clear yet although they are present in blood, vitreous fluid and in the extracellular matrix of vascular endothelial cells. A hypothesis that they might help FGF-2 release from cells is very interesting because FGF-2 does not have a clear secretion signal and the mechanism of the secretion of FGF-2 is still unclear. Single overexpression of FGF-2 is related neither to the secretion potential of the molecule nor to the tumourigenicity of the cells. In this report, BALB/c 3T3 cells transformed with the full length of human FGF-2 cDNA are further transformed with the cDNA coding the extracellular domain of human FGF receptor 1. The obtained transformants co-expressing FGF-2 and soluble FGF receptor are highly tumorigenic in nude mice, while the parental cells do not show any tumorigenicity. In the conditioned medium of the double-transformants, FGF-2 is immunologically detected. These results suggest that naturally produced soluble form of FGF receptor supports the release of FGF-2 from the cells and that over-expression of these two molecules leads to induce the malignant tumours in vivo. |
| Databáze: | OpenAIRE |
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