Quercetin treatment against ischemia/reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress

Autor:	Ilker Tinay, Selin Cadirci, Cem Akbal, D. Kıran, Göksel Şener, Şule Çetinel, Hasan Hüseyin Tavukçu, Tarik Emre Sener, Ozge Cevik
Přispěvatelé:	[Cevik, O.] Cumhuriyet Univ, Sch Pharm, Dept Biochem, Sivas, Turkey -- [Cadirci, S. -- Sener, G.] Marmara Univ, Sch Pharm, Dept Pharmacol, TR-34668 Istanbul, Turkey -- [Sener, T. E. -- Tinay, I. -- Akbal, C.] Marmara Univ, Sch Med, Dept Urol, TR-34668 Istanbul, Turkey -- [Tavukcu, H. H.] Acad Hosp, Dept Urol, Istanbul, Turkey -- [Cetinel, S. -- Kiran, D.] Marmara Univ, Sch Med, Dept Histol & Embryol, TR-34668 Istanbul, Turkey, Akbal, Cem -- 0000-0003-2202-6909, Cevik, Ozge -- 0000-0002-9325-3757, Sener, Tarik Emre -- 0000-0003-0085-7680
Rok vydání:	2013
Předmět:	Male antioxidant Ischemia Apoptosis Pharmacology Nitric Oxide medicine.disease_cause Biochemistry Antioxidants quercetin Nitric oxide Superoxide dismutase chemistry.chemical_compound corpus cavernosum medicine Animals biology apoptosis General Medicine Glutathione medicine.disease Malondialdehyde ischemia/reperfusion Rats Oxidative Stress chemistry inflammation Reperfusion Injury Myeloperoxidase Anesthesia biology.protein Quercetin Lipid Peroxidation Reperfusion injury Oxidative stress Penis
Zdroj:	Free Radical Research. 47:683-691
ISSN:	1029-2470 1071-5762 2375-8074
DOI:	10.3109/10715762.2013.814912
Popis:	WOS: 000323107900003 PubMed ID: 23758074 Reactive oxygen metabolites play an important role in the ischemia/reperfusion (I/R)-induced tissue injury. This study was designed to investigate the possible protective effects of quercetin against I/R injury of the rat corpus cavernosum tissue. To induce I/R injury, abdominal aorta was clamped for 30 min and reperfused for 60 min. Quercetin (20 mg/kg) or vehicle was given before ischemia and just after reperfusion in the I/R group and in the sham-operated control group in which clamping was not performed. After decapitation, corpus cavernosum tissues were removed and either placed in organ baths or stored for evaluating biochemical parameters. Oxidative injury was examined by measuring lucigenin chemiluminescence (CL), nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities and caspase-3 protein levels. In the I/R group, contractile responses to phenylephrine and relaxation responses to carbachol were impaired significantly compared with those in the control groups, while quercetin treatment in I/R group reversed both of the responses. On the other hand, increase in lucigenin CL, NO, MDA levels and MPO and caspase-3 activities and decrease in GSH levels and SOD activity in the cavernosal tissues of the I/R group were also significantly reversed by quercetin treatment. Furthermore, observed distorted morphology with ruptured endothelial cells and vacuolization in the cytoplasm of cavernosal tissues of I/R no longer persisted in the quercetin-treated I/R group. Thus, our results suggested that treatment with quercetin may have some benefits in controlling I/R-induced tissue injury through its anti-inflammatory, anti-apoptotic, and antioxidant effects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b037f9c15c1cdac3a24ba37ecd502565 https://doi.org/10.3109/10715762.2013.814912 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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