Resonant nonlinear microscopy reveals changes in molecular level chirality in native biological tissues
| Autor: | M.-Y. Chen, Mikko J. Huttunen, G. Deka, Yen-Yin Lin, Shi-Wei Chu, H.-L. Liu, C.-W. Kan, Ming-Jenn Wu, C.-W. Ye |
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| Přispěvatelé: | Tampere University, Photonics, Research group: Nonlinear Optics |
| Rok vydání: | 2018 |
| Předmět: |
Circular dichroism
Microscope Materials science 02 engineering and technology 01 natural sciences law.invention 010309 optics Optics Protein structure law 0103 physical sciences Microscopy Native state Electrical and Electronic Engineering Physical and Theoretical Chemistry Anisotropy business.industry Resolution (electron density) 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 216 Materials engineering Biophysics 0210 nano-technology business Chirality (chemistry) |
| Zdroj: | Optics Communications. 422:56-63 |
| ISSN: | 0030-4018 |
| DOI: | 10.1016/j.optcom.2018.03.005 |
| Popis: | Chirality is a fundamental property of biochemical molecules and often dictates their functionality. Conventionally, molecular chirality is studied by linear optical activity effects. However, poor contrast and artifacts due to anisotropy limit such studies to purified molecules not in their original microenvironments, potentially modifying their conformations. Here, we demonstrate that resonant second-harmonic-generation circular dichroism (SHG-CD) microscopy provides not only tissue imaging with improved chiral contrast, but also molecular chirality information of collagen, the most abundant protein in mammals, at its native state. Gradual protein denaturation shows that the resonant SHG-CD is dominated by the microscopic chirality related to collagen structures smaller than the spatial resolution of the microscope, i.e. to the protein conformation and microfibril organization, while the effects due to fiber orientation/anisotropy are mostly responsible of the non-resonant part. This result agrees well with a simple and intuitive model we propose to explain the resonant behavior and the consequent numerical SHG-CD simulations. Our results demonstrate the possibility to study molecular chirality in intact bio-tissues with nearly-unity contrast and sub-micrometer resolution, which will be useful in a broad range of biological and biochemical applications acceptedVersion |
| Databáze: | OpenAIRE |
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