Identification of a ‘Candidatus Phytoplasma hispanicum’-related strain, associated with yellows-type diseases, in smoke-tree sharpshooter (Homalodisca liturata Ball)
| Autor: | Diana Medina-Hernández, María Goretty Caamal-Chan, Alicia Chavez-Medina, Aarón Barraza, Rosalía Servín-Villegas, Abraham Loera-Muro, Ramón Jaime Holguín-Peña |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Phytoplasma Candidatus Phytoplasma hispanicum EcoRI 01 natural sciences Microbiology Hemiptera 03 medical and health sciences Phylogenetics RNA Ribosomal 16S Animals Mexico Gene Phylogeny Ecology Evolution Behavior and Systematics Plant Diseases Genetics biology Phylogenetic tree Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology biology.protein Restriction fragment length polymorphism Polymorphism Restriction Fragment Length 010606 plant biology & botany |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 68:2093-2101 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.002745 |
| Popis: | The 16SrXIII group from phytoplasma bacteria were identified in salivary glands from Homalodisca liturata, which were collected in El Comitan on the Baja California peninsula in Mexico. We were able to positively identify 15 16S rRNA gene sequences with the corresponding signature sequence of ‘Candidatus Phytoplasma ’ (CAAGAYBATKATGTKTAGCYGGDCT) and in silico restriction fragment length polymorphism (RFLP) profiles (F value estimations) coupled with a phylogenetic analysis to confirm their relatedness to ‘Candidatus Phytoplasma hispanicum’ , which in turn belongs to the 16SrXIII group. A restriction analysis was carried out with AluI and EcoRI to confirm that the five sequences belongs to subgroup D. The rest of the sequences did not exhibit any known RFLP profile related to a subgroup reported in the 16SrXIII group. |
| Databáze: | OpenAIRE |
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