Expression of ustR and the Golgi protease KexB are required for ustiloxin B biosynthesis in Aspergillus oryzae
| Autor: | Nozomi Nagano, Myco Umemura, Hideaki Koike, Masayuki Machida, Keietsu Abe, Akira Yoshimi |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Aspergillus oryzae Biophysics Aspergillus flavus Applied Microbiology and Biotechnology RiPS pathway Microbiology Secondary metabolite 03 medical and health sciences chemistry.chemical_compound Biosynthesis Gene cluster medicine Gene Protease biology Ustilaginoidea virens food and beverages Ribosomal RNA biology.organism_classification 030104 developmental biology Biochemistry chemistry Fungal C6-type transcription factor Ustiloxin B Original Article |
| Zdroj: | AMB Express |
| ISSN: | 2191-0855 |
| DOI: | 10.1186/s13568-016-0181-4 |
| Popis: | Ustiloxin B, originally isolated from the fungus Ustilaginoidea virens, is a known inhibitor of microtubule assembly. Ustiloxin B is also produced by Aspergillus flavus and is synthesized through the ribosomal peptide synthesis pathway. In A. flavus, the gene cluster associated with ustiloxin B production contains 15 genes including those encoding a fungal C6-type transcription factor and ustiloxin B precursor. Although the koji mold Aspergillus oryzae, which is genetically close to A. flavus, has the corresponding gene cluster, it does not produce ustiloxin B, which may be explained by the fact that the gene encoding the transcription factor UstR is not expressed. Here, to investigate whether ustiloxin B can be produced by expressing ustR in A. oryzae, we constructed ustR expression (ustREX) strains and analyzed ustiloxin B production. In the ustREX strains, all genes in the cluster were up-regulated, in line with expression of ustR, and ustiloxin B produced. To elucidate whether the KexB protease is involved in the processing of the ustiloxin B precursor protein UstA, which has repeats of basic amino acid doublets resembling KexB target sites, we also constructed a ustREX strain with the ∆kexB genotype. Although ustR was expressed in this strain, ustiloxin B was barely detectable. This finding strongly suggests that KexB is required for ustiloxin B production. Electronic supplementary material The online version of this article (doi:10.1186/s13568-016-0181-4) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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