Synergism of interleukin-12 and interleukin-3 on development of hematopoietic progenitors
| Autor: | M. Dzondo‐Gadet, J. P. Vannier, G. Teixeira‐Lebrun, B. Lenormand, D. Fardoun‐Joalland |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Myeloid
medicine.medical_treatment Cell Biology medicine Humans Progenitor cell Interleukin 3 Erythroid Precursor Cells Dose-Response Relationship Drug Interleukin-6 Growth factor Granulocyte-Macrophage Colony-Stimulating Factor Drug Synergism Hematology General Medicine Hematopoietic Stem Cells Interleukin-12 Cell biology Hematopoiesis Haematopoiesis medicine.anatomical_structure Immunology Interleukin 12 Interleukin-3 Stem cell |
| Zdroj: | European journal of haematology. 54(3) |
| ISSN: | 0902-4441 |
| Popis: | The recently cloned cytotoxic lymphocyte maturation factor [CLMF] also called NK cell stimulatory factor [NKSF] or interleukin-12 [IL-12] has been described as a growth factor for mature lymphoid cells. The present study investigated whether purified recombinant human IL-12 could stimulate CFU colony growth. Source of progenitor cells were peripheral blood cells depleted of adherent, CD2- and CD56-positive cells. RhIL-12 was investigated either alone or in combination with rhIL-3, rhIL-6 and rhGM-CSF. RhIL-12 alone did not support colony formation of myeloid or erythroid progenitors. RhIL-12 in combination with rhIL-3 increased the numbers of BFU-E and CFU-GM. No synergism or additive effect was seen with the combination of rhIL-12 and rhGM-CSF or rhIL-12 and rhIL-6. An additive increase in the number of granulocytic colonies was observed when rhIL-3, rhIL-6 and rhGM-CSF were used together with rhIL-12. Our result therefore suggest that, in addition to being a potent lymphopoietic stimulator, IL-12 acts synergistically with IL-3 in enhancing the sensitivity of hemopoietic progenitors to IL-3. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text