Stimulated release of acetylcholinesterase in rat striatum revealed by in vivo microspectrophotometry
| Autor: | P. Gourmelon, D. Clarençon, Guy Testylier, M. Fatome, Jacques Viret, M. Estrade, M. Galonnier |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Central nervous system Stimulation Striatum chemistry.chemical_compound In vivo Internal medicine medicine Extracellular Animals Rats Wistar biology General Neuroscience Acetylcholinesterase Corpus Striatum Electric Stimulation Enzyme assay Rats Kinetics medicine.anatomical_structure Endocrinology chemistry Spectrophotometry Potassium biology.protein Biophysics Intracellular |
| Zdroj: | Neuroscience. 55:457-462 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/0306-4522(93)90515-h |
| Popis: | The microspectrophotometric technique allows a direct in vivo measurement of brain extracellular acetylcholinesterase. An optical probe associated with electrodes for stimulation was implanted in striatum of anaesthetized rats to determine the effects of neuronal excitation on the acetylcholinesterase activity. Electrical stimulations induced a reversible increase in acetylcholinesterase activity of about 30 to 50%, with a recovery to baseline occurring after 1 or 2 h. Furthermore, iterative electrical stimulation induced a progressive fading of this phenomenon. An enhancement of acetylcholinesterase activity was also observed by stimulations with potassium injections through a canal of the probe. These results suggest mainly an intracellular origin of the released enzyme and estimate its contribution at about 40% of the whole extracellular enzyme activity. |
| Databáze: | OpenAIRE |
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