SDP1 is a peroxisome-proliferator-activated receptor gamma2 co-activator that binds through its SCAN domain
| Autor: | Robert M. Babb, Benjamin R. Bowen |
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| Rok vydání: | 2003 |
| Předmět: |
Molecular Sequence Data
Receptors Cytoplasmic and Nuclear Sequence Homology Peroxisome proliferator-activated receptor ZNF202 In Vitro Techniques Biochemistry Humans Amino Acid Sequence Receptor Molecular Biology Transcription factor chemistry.chemical_classification biology Intracellular Signaling Peptides and Proteins Lipid metabolism Cell Biology Transfection Blotting Northern Protein Structure Tertiary Nuclear receptor chemistry Trans-Activators biology.protein lipids (amino acids peptides and proteins) PPARGC1A HeLa Cells Transcription Factors Research Article |
| Zdroj: | Biochemical Journal. 370:719-727 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj20021378 |
| Popis: | Peroxisome-proliferator-activated receptors (PPARs), members of the nuclear hormone receptor superfamily, play an important role in the regulation of lipid metabolism and energy homoeostasis. In a yeast two-hybrid experiment using the zinc-finger transcription factor ZNF202 as bait, we previously identified the SCAN-domain-containing protein SDP1. SDP1 shares a high degree of amino acid sequence identity with PGC-2, a previously identified PPAR gamma 2 co-activator from the mouse. Here we show that SDP1 and PGC-2 interact with PPAR gamma 2 through their SCAN domains, even though PPAR gamma 2 does not contain a SCAN domain. Similar to PGC-2, SDP1 enhanced PPAR gamma 2-dependent gene transcription in transiently transfected cells but did not alter the affinity of PPAR gamma 2 for agonists. Although the SCAN domain was necessary for binding to PPAR gamma 2, it was not sufficient for co-activation in cells, suggesting that other features of SDP1 are responsible for transcriptional co-activation. The ability of SDP1 to interact with two different transcription factors that regulate genes involved in lipid metabolism, ZNF202 and PPAR gamma 2, suggests that SDP1 may be an important co-regulator of such genes. |
| Databáze: | OpenAIRE |
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