Respiratory epithelial permeability in complete Freund's adjuvant rat lung granulomatosis
| Autor: | Mordelet-Dambrine Ms, D Henzel, G Huchon, Barritault L, G Stanislas-Leguern |
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| Rok vydání: | 1994 |
| Předmět: |
Lung Diseases
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Cell Membrane Permeability Time Factors Freund's Adjuvant Critical Care and Intensive Care Medicine complex mixtures Epithelium Alveolar cells medicine Animals Rats Wistar Respiratory system Radionuclide Imaging Lung Horseradish Peroxidase Granuloma medicine.diagnostic_test business.industry respiratory system medicine.disease Rats Specific Pathogen-Free Organisms Disease Models Animal medicine.anatomical_structure Bronchoalveolar lavage Peroxidases Freund's adjuvant Technetium Tc 99m Pentetate Respiratory epithelium business Bronchoalveolar Lavage Fluid |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 150:1660-1666 |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/ajrccm.150.6.7952629 |
| Popis: | To investigate Freund's adjuvant-induced changes in respiratory epithelial permeability, we determined the respiratory epithelium clearance of 99mTc-DTPA (RC-DTPA) in rats and the localization of horseradish peroxidase (HRP) across the lung epithelium. RC-DTPA was determined before and after intravenous injection of saline (control) or complete Freund's adjuvant (CFA). Although the respiratory epithelium was not disrupted, RC-DTPA was increased 15 min after injection of CFA and was still high after 3 d but had returned to normal after 7 d, after which it again increased and stayed high from Day 42 through Day 112. Cell number obtained by bronchoalveolar lavage increased from 15 min to 3 d after injection of CFA. Lung granulomatosis was observed 14 to 112 d after injection of CFA; changes in RC-DTPA correlated with changes in alveolar cells. HRP was present in bronchiolar intercellular spaces only in the CFA group. We conclude that (1) RC-DTPA increases in rat lung CFA granulomatosis 15 min to 3 and 42 to 112 d following injection, (2) the increase in RC-DTPA is not related to a rupture of epithelium but (3) is proportional to the cell influx into the lung, and (4) is possibly caused by the opening of tight junctions in small airways. |
| Databáze: | OpenAIRE |
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