Inactivation of cellobiose dehydrogenases modifies the cellulose degradation mechanism of Podospora anserina
| Autor: | David Navarro, Kevin D. Hyde, Valérie Gautier, Didier Chevret, Laetitia Chan Ho Tong, Narumon Tangthirasunun, Philippe Silar, Jean-Guy Berrin, Sona Garajova |
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| Přispěvatelé: | Laboratoire Interdisciplinaire des Energies de Demain (LIED (UMR_8236)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Biodiversité et Biotechnologie Fongiques (BBF), École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Center of Excellence in Fungal Research and School of Science, Mae Fah Luang University [Thaïlande] (MFU), Université Paris Diderot - Paris 7 (UPD7), grant P3AMB Region Ile de France, Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM), INL - Conception de Systèmes Hétérogènes (INL - CSH), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut Jacques Monod (IJM (UMR_7592)), Ambassade de France à Bangkok University Paris 7Région Ile-de-FranceP3AMBUniversity Paris 11, Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellobiose dehydrogenase dégradation de la biomasse [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV]Life Sciences [q-bio] Mutant Cellobiose Polysaccharide Applied Microbiology and Biotechnology Podospora anserina Fungal Proteins 03 medical and health sciences chemistry.chemical_compound podospora anserina Podospora dégradation de la cellulose Cellulose cellobiose dehydrogenase Phylogeny 2. Zero hunger chemistry.chemical_classification Ecology biology beta glucosidase Beta-glucosidase Wild type biomass degradation 15. Life on land biology.organism_classification Enzyme Activation 030104 developmental biology Phenotype Biochemistry chemistry cellobiose déshydrogénase Biodegradation Carbohydrate Dehydrogenases Gene Deletion Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2016, 83 (2), ⟨10.1128/AEM.02716-16⟩ Applied and Environmental Microbiology, American Society for Microbiology, 2016, 83 (2), pp.e02716. ⟨10.1128/AEM.02716-16⟩ Applied and Environmental Microbiology, American Society for Microbiology, 2017, 83 (2), pp.e02716. ⟨10.1128/AEM.02716-16⟩ Applied and Environmental Microbiology, 2016, 83 (2), pp.e02716. ⟨10.1128/AEM.02716-16⟩ |
| ISSN: | 0099-2240 1098-5336 |
| Popis: | Conversion of biomass into high-value products, including biofuels, is of great interest to developing sustainable biorefineries. Fungi are an inexhaustible source of enzymes to degrade plant biomass. Cellobiose dehydrogenases (CDHs) play an important role in the breakdown through synergistic action with fungal lytic polysaccharide monooxygenases (LPMOs). The three CDH genes of the model fungus Podospora anserina were inactivated, resulting in single and multiple CDH mutants. We detected almost no difference in growth and fertility of the mutants on various lignocellulose sources, except on crystalline cellulose, on which a 2-fold decrease in fertility of the mutants lacking P. anserina CDH1 ( PaCDH1 ) and PaCDH2 was observed. A striking difference between wild-type and mutant secretomes was observed. The secretome of the mutant lacking all CDHs contained five beta-glucosidases, whereas the wild type had only one. P. anserina seems to compensate for the lack of CDH with secretion of beta-glucosidases. The addition of P. anserina LPMO to either the wild-type or mutant secretome resulted in improvement of cellulose degradation in both cases, suggesting that other redox partners present in the mutant secretome provided electrons to LPMOs. Overall, the data showed that oxidative degradation of cellulosic biomass relies on different types of mechanisms in fungi. IMPORTANCE Plant biomass degradation by fungi is a complex process involving dozens of enzymes. The roles of each enzyme or enzyme class are not fully understood, and utilization of a model amenable to genetic analysis should increase the comprehension of how fungi cope with highly recalcitrant biomass. Here, we report that the cellobiose dehydrogenases of the model fungus Podospora anserina enable it to consume crystalline cellulose yet seem to play a minor role on actual substrates, such as wood shavings or miscanthus. Analysis of secreted proteins suggests that Podospora anserina compensates for the lack of cellobiose dehydrogenase by increasing beta-glucosidase expression and using an alternate electron donor for LPMO. |
| Databáze: | OpenAIRE |
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