Remodeling Chromatin Induces Z-DNA Conformation Detected through Fourier Transform Infrared Spectroscopy
| Autor: | Guohua Yao, Qing Huang, Xiaoyi Wang, Xin-Xin Zheng, Chang-Sheng Shao, Xumiao Jing, Fengqiu Zhang, Huiyue Zhou, Tao Wang, Yalin Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
animal structures
Protein subunit 010402 general chemistry 01 natural sciences Chromatin remodeling Analytical Chemistry Z-DNA chemistry.chemical_compound Adenosine Triphosphate Cell Line Tumor Spectroscopy Fourier Transform Infrared DNA Z-Form Humans Fourier transform infrared spectroscopy Gene knockdown Chemistry 010401 analytical chemistry DNA Helicases Nuclear Proteins Chromatin Assembly and Disassembly 0104 chemical sciences Chromatin Transformation (genetics) Gene Knockdown Techniques Biophysics Nucleic Acid Conformation DNA Transcription Factors |
| Zdroj: | Analytical chemistry. 92(21) |
| ISSN: | 1520-6882 |
| Popis: | The SWI/SNF complex is a highly conserved chromatin remodeling complex and can hydrolyze ATP by its catalytic subunit BRG1 or BRM to reconstruct the chromatin. To investigate whether this ATP-dependent chromatin remodeling could affect the DNA conformation, we therefore regulated (knocked down or overexpressed) BRG1/BRM in the cells and applied Fourier transform infrared (FTIR) spectroscopy to probe DNA conformational changes. As a result, we found that BRG1/BRM was indeed associated with the DNA conformational changes, in which knockdown of BRG1/BRM reduced Z-DNA conformation, while overexpression of BRG1/BRM enhanced Z-DNA conformation. This Z-DNA conformational transformation was also verified using the Z-DNA-binding proteins. Therefore, this work has provided a direct analytical tool to probe Z-DNA transformation upon ATP-dependent chromatin remodeling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|