hElp3 Directly Modulates the Expression of HSP70 Gene in HeLa Cells via HAT Activity
| Autor: | Shu-juan Tian, Yanyan Hu, Richard E. White, Yu Ma, Ji-Xian Ma, Fen Li, Guichun Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Chromatin Immunoprecipitation
Science DNA transcription Blotting Western Biophysics Gene Expression Down-Regulation Nerve Tissue Proteins Biology Real-Time Polymerase Chain Reaction Biochemistry Molecular Genetics Molecular cell biology Transcription (biology) Gene expression Transcriptional regulation Genetics Humans HSP70 Heat-Shock Proteins Northern blot RNA Messenger RNA synthesis Gene DNA Primers Histone Acetyltransferases Regulation of gene expression Messenger RNA Multidisciplinary Base Sequence Physics Genetic Complementation Test Computational Biology Blotting Northern Molecular biology Nucleic acids Medicine RNA Chromatin immunoprecipitation Research Article HeLa Cells |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 12, p e29303 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed hELP3 expression, and substantially reduced expression of HSP70 mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of HSPA1A in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of HSP70 (SSA3) gene transcription, but also directly regulates the transcription of SSA3. On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of hELP3 in human cells. |
| Databáze: | OpenAIRE |
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