The Coagulant Factor Xa Induces Protease-Activated Receptor-1 and Annexin A2-Dependent Airway Smooth Muscle Cytokine Production and Cell Proliferation
| Autor: | Michael Schuliga, Shenna Y. Langenbach, Trudi Harris, Christine R. Keenan, Asres Berhan, Simon G. Royce, Alastair G. Stewart |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine medicine.medical_specialty Time Factors medicine.medical_treatment Biopsy Clinical Biochemistry Myocytes Smooth Muscle Respiratory System Biology Pharmacology Transfection 03 medical and health sciences chemistry.chemical_compound Thrombin Internal medicine medicine Humans Pyrroles Receptor PAR-1 RNA Messenger Receptor Molecular Biology Annexin A2 Cells Cultured Cell Proliferation Dose-Response Relationship Drug Cell growth Factor X Integrin beta1 Cell Biology Asthma respiratory tract diseases CTGF 030104 developmental biology Cytokine Endocrinology chemistry Gene Expression Regulation Factor Xa Quinazolines Airway Remodeling Cytokines RNA Interference Signal transduction medicine.drug Signal Transduction |
| Zdroj: | American journal of respiratory cell and molecular biology. 54(2) |
| ISSN: | 1535-4989 |
| Popis: | During asthma exacerbation, plasma circulating coagulant factor X (FX) enters the inflamed airways and is activated (FXa). FXa may have an important role in asthma, being involved in thrombin activation and an agonist of protease-activated receptor-1 (PAR-1). Extracellular annexin A2 and integrins are also implicated in PAR-1 signaling. In this study, the potential role of PAR-1 in mediating the effects of FXa on human airway smooth muscle (ASM) cell cytokine production and proliferation was investigated. FXa (5-50 nM), but not FX, stimulated increases in ASM IL-6 production and cell number after 24- and 48-hour incubation, respectively (P < 0.05; n = 5). FXa (15 nM) also stimulated increases in the levels of mRNA for cytokines (IL-6), cell cycle-related protein (cyclin D1), and proremodeling proteins (FGF-2, PDGF-B, CTGF, SM22, and PAI-1) after 3-hour incubation (P |
| Databáze: | OpenAIRE |
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