A Highly Expressed Human Protein, Apolipoprotein B-100, Serves as an Autoantigen in a Subgroup of Patients With Lyme Disease
| Autor: | Allen C. Steere, Elise E. Drouin, Catherine E. Costello, Klemen Strle, Jameson T. Crowley, Annalisa Pianta, Qi Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Apolipoprotein B
T-Lymphocytes Human leukocyte antigen Lyme Arthritis medicine.disease_cause Autoantigens Epitope Autoimmunity Cohort Studies Major Articles and Brief Reports medicine Humans Immunology and Allergy Borrelia burgdorferi B-Lymphocytes Lyme Disease biology Autoantibody Middle Aged biology.organism_classification Infectious Diseases medicine.anatomical_structure Apolipoprotein B-100 Immunology biology.protein Female Synovial membrane |
| Zdroj: | Journal of Infectious Diseases. 212:1841-1850 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | To discover novel autoantigens associated with Lyme arthritis (LA), we identified T-cell epitopes presented in vivo by human leukocyte antigen (HLA)-DR molecules in patients' inflamed synovial tissue or joint fluid and tested each epitope for autoreactivity. Using this approach, we identified the highly expressed human protein, apolipoprotein B-100 (apoB-100), as a target of T- and B-cell responses in a subgroup of LA patients. Additionally, the joint fluid of these patients had markedly elevated levels of apoB-100 protein, which may contribute to its autoantigenicity. In patients with antibiotic-refractory LA, the magnitude of apoB-100 antibody responses correlated with increased numbers of plasma cells in synovial tissue, greater numbers and activation of endothelial cells, and more synovial fibroblast proliferation. Thus, a subset of LA patients have high levels of apoB-100 in their joints and autoreactive T- and B-cell responses to the protein, which likely contributes to pathogenic autoimmunity in patients with antibiotic-refractory LA. |
| Databáze: | OpenAIRE |
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