Synthetic G-Quartets as Versatile Nanotools for the Luminescent Detection of G-Quadruplexes
| Autor: | Fares Katranji, Marc Pirrotta, Loic Stefan, David Monchaud, Marine Levillain, Aurélien Laguerre, Romain Haudecoeur |
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| Přispěvatelé: | Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de pharmacochimie moléculaire (DPM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Conception, synthèse et vectorisation de biomolécules. (CSVB), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris], Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Luminescence Guanine Supramolecular chemistry Nanotechnology G-quadruplex Tasq Nucleobase chemistry.chemical_compound Dota Organometallic Compounds Nanobiotechnology [CHIM]Chemical Sciences Luminescent Agents Terbium QD1-999 ComputingMilieux_MISCELLANEOUS Molecular switch Chemistry Pyrene General Medicine General Chemistry Thymine G-Quadruplexes Drug Design Luminescent Measurements Graphite |
| Zdroj: | CHIMIA International Journal for Chemistry CHIMIA International Journal for Chemistry, 2015, 69 (9), pp.530-536. ⟨10.2533/chimia.2015.530⟩ CHIMIA, Vol 69, Iss 9 (2015) |
| ISSN: | 0009-4293 |
| DOI: | 10.2533/chimia.2015.530⟩ |
| Popis: | Recent years have witnessed a tremendous increase in the biotechnological applications of nucleic acid-based nanotools. Beyond their biological relevance, nucleobases have indeed found new scopes of applications in bionanotechnology, which are expanding nowadays at an accelerated pace. Among the four canonical nucleobases (adenine, guanine, cytosine and thymine), guanine is certainly the most useful and used base, thanks to its versatile H-bond donating/accepting properties that make it suitable for being involved in various assemblies ranging from base-pairs to base-quartets. Here, we would like to report on an innovative guanine-based molecular tool named Tb. Pyro-DOTASQ: this metal complex has a sophisticated chemical structure that allows formation of an intramolecular G-quartet upon interaction with alternative secondary structures known as G-quadruplexes. This target-promoted molecular switch triggers a luminescence response that would permit the use of Tb. Pyro-DOTASQ to search and detect quadruplex-forming DNA and RNA sequences: its unique design indeed allows it i) to create specific interaction with quadruplexes, ii) to provide an easily readable luminescent output to monitor this association and iii) to be readily immobilized on graphene surface, thus making Tb. Pyro-DOTASQ a high-value molecular device. Results obtained in the course of in-depth biophysical analyses raise questions about the actual supramolecular structure of Tb. Pyro-DOTASQ: these results thus shed a bright light on the care that must be exercised when using intricate molecular architectures to construct elaborated supramolecular metal complexes. |
| Databáze: | OpenAIRE |
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