Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro
| Autor: | Sonia Abou Najem, Robin I. Taleb, Marianne El Khoury, Tony Haykal, Mohammad Hassan Hodroj, Sandra Rizk, Rita Sarkis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Poly ADP ribose polymerase Malva pseudolavatera Webb & Berthel acute myeloid leukemia lcsh:RC254-282 Article 03 medical and health sciences Phytol chemistry.chemical_compound 0302 clinical medicine Malva Malva pseudolavatera Webb & Berthel acids chemistry.chemical_classification reactive oxygen species malva pseudolavatera webb & berthel Reactive oxygen species plants Chemistry apoptosis Myeloid leukemia lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Molecular biology Quercitrin polymerase 030104 developmental biology Oncology Apoptosis 030220 oncology & carcinogenesis cytotoxicity DNA fragmentation activation |
| Zdroj: | Cancers Volume 12 Issue 2 Cancers, Vol 12, Iss 2, p 435 (2020) |
| ISSN: | 2072-6694 |
| Popis: | Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on M. pseudolavatera Webb & Berthel. leaves have documented anti-inflammatory and anti-oxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of Malva pseudolavatera Webb & Berthel. leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with M. pseudolavatera methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species (ROS) as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, andrelease of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, &gamma sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry, and amino acids, amino acid derivatives, tiliroside, 13-hydroxyperoxyoctadecadienoic, and quercitrin as detected by liquid chromatography coupled to mass spectrometry. |
| Databáze: | OpenAIRE |
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