Fluorescence polarization immunoassays for the quantification of caffeine in beverages
| Autor: | Leif-Alexander Garbe, Julia Grandke, Ute Resch-Genger, Rudolf J. Schneider, Lidia Oberleitner, Frank Mallwitz |
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| Rok vydání: | 2014 |
| Předmět: |
Chromatography
Analytical chemistry General Chemistry Cosmetics Decaffeinated coffee Cuvette Beverages chemistry.chemical_compound chemistry Homogeneous Caffeine Fluorescence Polarization Immunoassay Fluorescence polarization immunoassay General Agricultural and Biological Sciences Quantitative analysis (chemistry) Fluorescence anisotropy |
| Zdroj: | Journal of agricultural and food chemistry. 62(11) |
| ISSN: | 1520-5118 |
| Popis: | Homogeneous fluorescence polarization immunoassays (FPIAs) were developed and compared for the determination of caffeine in beverages and cosmetics. FPIAs were performed in cuvettes in a spectrometer for kinetic FP measurements as well as in microtiter plates (MTPs) on a multimode reader. Both FPIAs showed measurement ranges in the μg/L range and were performed within 2 and 20 min, respectively. For the application on real samples, high coefficients of variations (CVs) were observed for the performance in MTPs; the CVs for FPIAs in cuvettes were below 4%. The correlations between this method and reference methods were satisfying. The sensitivity was sufficient for all tested samples including decaffeinated coffee without preconcentration steps. The FPIA in cuvettes allows a fast, precise, and automated quantitative analysis of caffeine in consumer products, whereas FPIAs in MTPs are suitable for semiquantitative high-throughput screenings. Moreover, specific quality criteria for heterogeneous assays were applied to homogeneous immunoassays. |
| Databáze: | OpenAIRE |
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