Embryonic poly(A)-binding protein is required at the preantral stage of mouse folliculogenesis for oocyte–somatic communication†
| Autor: | Emre Seli, Cai-Rong Yang, Katie M. Lowther, Hugh S. Taylor, Federico Favero |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system Somatic cell Ovary Cell Communication Biology Poly(A)-Binding Protein I Poly(A)-Binding Proteins Connexins Andrology Mice 03 medical and health sciences Ovarian Follicle medicine Animals Ovarian follicle Mice Knockout Gap junction Gap Junctions Cell Biology General Medicine Anatomy Oocyte Antral follicle Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Reproductive Medicine Connexin 43 Oocytes Female Folliculogenesis |
| Zdroj: | Biology of Reproduction. 96:341-351 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.116.141234 |
| Popis: | Embryonic poly(A)-binding protein (EPAB)-deficient mice are infertile due to defects in both the oocyte and the somatic cells of the ovary. Since EPAB is oocyte specific, the abnormalities in the somatic compartment of Epab−/− mice are likely due to factors inherent to the oocyte. Herein, we investigated whether oocyte–somatic communication is disrupted as a result of EPAB deficiency. We found that gap junctions are disrupted at the late preantral stage of folliculogenesis in Epab−/– mice and remain disrupted in cumulus-enclosed oocytes (COCs) from antral follicles. Consistent with the timing of gap junction dysfunction, F-actin staining of transzonal processes (TZPs) is lower in Epab−/− follicles at the late preantral stage and completely absent in Epab−/− COCs. Epab−/− oocytes express significantly lower levels of the junction protein E-cadherin, which is likely to be a contributing factor leading to premature TZP retraction. Overall, these results demonstrate that EPAB is important for oocyte–somatic communication by maintaining TZPs and gap junctions at the preantral stage of folliculogenesis. |
| Databáze: | OpenAIRE |
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