Visualization of Cellular Components in a Mammalian Cell with Liquid-Cell Transmission Electron Microscopy
| Autor: | Günther Kassier, Robert Bücker, Deybith Venegas-Rojas, Andrea Rentmeister, Stephanie Besztejan, Stephanie Manz, R. J. Dwayne Miller, Sercan Keskin, Hoc Khiem Trieu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Glutamate Carboxypeptidase II Male Materials science Nucleolus Cell Microfluidics Analytical chemistry Cell Culture Techniques Electrons Cell membrane 03 medical and health sciences Microscopy Electron Transmission Cell Line Tumor LNCaP medicine Image Processing Computer-Assisted Humans Instrumentation Staining and Labeling Vesicle Silicon Compounds Prostatic Neoplasms 030104 developmental biology medicine.anatomical_structure Transmission electron microscopy Colloidal gold Antigens Surface Biophysics Microtechnology Nanoparticles Intracellular |
| Zdroj: | Microscopy and Microanalysis |
| ISSN: | 1435-8115 |
| Popis: | We present liquid-cell transmission electron microscopy (liquid-cell TEM) imaging of fixed and non-fixed prostate cancer cells (PC3 and LNCaP) with high resolution in a custom developed silicon nitride liquid cell. Fixed PC3 cells were imaged for 90–120 min without any discernable damage. High contrast on the cellular structures was obtained even at low electron doses (~2.5 e−/nm2 per image). The images show distinct structures of cell compartments (nuclei and nucleoli) and cell boundaries without any further sample embedding, dehydration, or staining. Furthermore, we observed dynamics of vesicles trafficking from the cell membrane in consecutive still frames in a non-fixed cell. Our findings show that liquid-cell TEM, operated at low electron dose, is an excellent tool to investigate dynamic events in non-fixed cells with enough spatial resolution (few nm) and natural amplitude contrast to follow key intracellular processes. |
| Databáze: | OpenAIRE |
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