Dissolution of type I collagen fibrils by gingival fibroblasts isolated from patients of various periodontitis categories
| Autor: | Palle Holmstrup, Henning Birkedal-Hansen, Anne Havemose-Poulsen, Kaj Stoltze |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Time Factors Adolescent medicine.medical_treatment Gingiva Periodontal Attachment Loss Biopsy medicine Humans Aggressive periodontitis Collagenases Child Periodontitis Fibroblast Cells Cultured Aged Analysis of Variance medicine.diagnostic_test Chemistry Age Factors Metalloendopeptidases Fibroblasts Middle Aged medicine.disease medicine.anatomical_structure Cytokine Aggressive Periodontitis Clinical attachment loss Carcinogens Disease Progression Collagenase Regression Analysis Tetradecanoylphorbol Acetate Periodontics Female Collagen Disease Susceptibility Type I collagen Interleukin-1 medicine.drug |
| Zdroj: | Journal of Periodontal Research. 33:280-291 |
| ISSN: | 1600-0765 0022-3484 |
| DOI: | 10.1111/j.1600-0765.1998.tb02321.x |
| Popis: | The classification of periodontitis in various disease categories, including juvenile periodontitis, rapidly progressive adult periodontitis and slowly progressive adult periodontitis is based mainly on differences in disease progression and age group susceptibility. Because dissolution of collagen fibers is an integral part of periodontal attachment loss, we investigated whether the clinical differences among these periodontitis/control groups are reflected in the collagen-degrading activity of gingival fibroblasts isolated from affected tissues. All fibroblast strains isolated from the 4 groups (n = 48) displayed cell-associated collagenolytic activity when seeded in contact with a reconstituted film of type I collagen fibrils. Cells from the control group (n = 14) dissolved the collagen fibril film twice as fast as those from each of the 3 disease groups (juvenile periodontitis (n = 13), rapidly progressive adult periodontitis (n = 7), and slowly progressive adult periodontitis (n = 14)). Both interleukin-1 beta and phorbolester accelerated the rate of dissolution 2-4-fold, but even after cytokine or phorbolester stimulation control cells were still considerably more effective in dissolving the collagen fibrils than cells from the disease groups. The observation made in this study, that dissolution of collagen fibrils by gingival fibroblasts from periodontally diseased individuals is significantly slower than by cells from healthy control subjects, challenges disease paradigms based on a direct relationship between collagenolytic potential and disease activity. |
| Databáze: | OpenAIRE |
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