Evaluation of Metabolic Alteration in Transgenic Rice Overexpressing Dihydroflavonol-4-reductase
| Autor: | Fumiyuki Goto, Mitsunori Hayashi, Hirofumi Uchimiya, Hideyuki Takahashi, Maki Kawai-Yamada, Tomoyoshi Soga, Shigeru Sato, Takaaki Nishioka, Masaru Tomita |
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| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_classification
Glutathione peroxidase Glutathione reductase food and beverages Oryza Original Articles Plant Science Glutathione Tricarboxylic acid Pentose phosphate pathway Biology Plants Genetically Modified Plant Roots Genetically modified rice Amino acid Plant Leaves Alcohol Oxidoreductases chemistry.chemical_compound chemistry Biochemistry Gene Expression Regulation Plant Seeds Metabolome Amino Acids |
| Zdroj: | Annals of Botany. 98:819-825 |
| ISSN: | 1095-8290 0305-7364 |
| Popis: | • Background and Aims Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress tolerance. High throughput profiles of metabolites have also been shown in such transgenic plants by Fourier transform ion cyclotron mass spectrometry. In this study, capillary electrophoresis mass spectrometry analysis (CE/MS) was employed to identify precise metabolites such as organic acids, amino acids and sugars. • Methods Using CE/MS, we analysed several metabolites of glycolysis, the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. In addition, the concentrations of sugars and ion were quantified. • Key Results In YK1 (DFR)-overexpressing plants, the concentrations of cis-aconitate, isocitrate and 2-oxoglutarate were higher in leaves, whereas those of fructose-1,6-bisphosphate and glyceraldehyde-3-phosphate were lower in roots. In seeds, the amounts of free amino acids and metals were altered, whereas sugars in seeds were kept constant. In YK1 calli, an approx. 3-fold increase in glutathione was observed, whereas the activities of glutathione peroxidase and glutathione reductase were concomitantly increased. • Conclusions The overexpression of YK1 (DFR) was associated with slight changes in the amounts of several metabolites analysed in whole plants, whilst glutathione derivatives were substantially increased in suspension-cultured cells. |
| Databáze: | OpenAIRE |
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