Mas receptor overexpression increased Ang-(1–7) relaxation response in renovascular hypertensive rat carotid
| Autor: | Rosária Dias Aires, Leandra Naira Zambelli Ramalho, Steyner F. Cortes, Virginia S. Lemos, L. B. Santiago, V. C. Olivon |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Nitric Oxide Synthase Type III Physiology Ibuprofen Vasodilation Nitric Oxide Proto-Oncogene Mas Biochemistry Receptors G-Protein-Coupled Nitric oxide Renovascular hypertension Renin-Angiotensin System Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Enos Proto-Oncogene Proteins Internal medicine Renin–angiotensin system ÓXIDO NÍTRICO medicine Animals Rats Wistar Receptor biology biology.organism_classification medicine.disease Peptide Fragments Rats Carotid Arteries NG-Nitroarginine Methyl Ester Gene Expression Regulation chemistry Pathophysiology of hypertension Hypertension cardiovascular system Angiotensin I |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Renin-angiotensin system (RAS) is an important factor in the pathophysiology of hypertension. Mas receptor, Angiotensin-(1-7) [Ang-(1-7)]-activated receptor, is an important RAS component and exerts protective effects in the vasculature. Ang-(1-7) vascular effects and Mas receptor expression in carotid from renovascular hypertensive (2K-1C) rats is not clear. In the present study we investigated Mas receptor vasodilator response activated by Ang-(1-7) in the carotid rings from sham and 2K-1C rats. Changes in isometric tension were recorded on organ chamber. Mas receptors expression was investigated in carotid by Western blot. Nitric oxide production was evaluated by 2,3-diaminonaphthalene (DAN) and eNOS expression and activity by immunofluoresce and western blot, respectively. Ang-(1-7) induced concentration-dependent vasodilator effect in carotid rings from sham and 2K-1C, which the hypertension increased vasodilatation response. In the 2K-1C carotid rings, A-779 (Mas receptor antagonist) reduced but not abolish the vasodilator effect of Ang-(1-7). Corroborating, Mas receptor protein expression was significantly increased in the 2K-1C rats. L-NAME and ibuprofen decreased Ang-(1-7) vasodilator response and L-NAME plus ibuprofen practically abolish the remaining vasodilatation response. Nitric oxide production is increased due increased of eNOS expression and pSer(1177) activity. Our results demonstrated that renovascular hypertension increased Mas receptors expression and nitric oxide production in the rats carotid which, consequently increased Ang-(1-7)-vasorelaxant response. |
| Databáze: | OpenAIRE |
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