Effect of advanced glycation end products on platelet activation and aggregation: a comparative study of the role of glyoxal and methylglyoxal
| Autor: | Cristian Arriagada-Petersen, Paula Fernandez, Eduardo Fuentes, Maira Gomez, Felipe Ávila, Natalia Ravello, Iván Palomo |
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| Rok vydání: | 2020 |
| Předmět: |
Glycation End Products
Advanced 0301 basic medicine medicine.medical_specialty Platelet Aggregation P-selectin 030204 cardiovascular system & hematology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glycation Internal medicine medicine Humans Platelet Platelet activation Dietary intake Methylglyoxal Glyoxal Hematology General Medicine Platelet Activation Pyruvaldehyde 030104 developmental biology Endocrinology chemistry |
| Zdroj: | Platelets. 32:507-515 |
| ISSN: | 1369-1635 0953-7104 |
| DOI: | 10.1080/09537104.2020.1767770 |
| Popis: | Advanced glycation end products (AGEs) arising from dietary intake have been associated with numerous chronic diseases including cardiovascular diseases. The interaction between platelets and AGEs has been proposed to play a role in the etiology of cardiovascular diseases. However, the effects of the interaction between platelets and Maillard reaction products generated from glyoxal (Gly) or methylglyoxal (MG) are poorly understood. In this work, the effects of AGEs generated by the reaction between Gly or MG with Lys or bovine serum albumin (BSA) on platelet activation and aggregation were assessed. AGEs were generated incubating Gly or MG with Lys or BSA during 5 hours or 14 days, respectively. AGEs generation were characterized by kinetic studies and by amino acid analysis. Human platelet-rich plasma (PRP) was incubated with different concentrations of AGEs from Lys-MG or Lys-Gly and BSA-MG or BSA-Gly. Platelet activation was determined quantifying the expression of CD62 ( |
| Databáze: | OpenAIRE |
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