Solving an Old Dogma: Is it an Arteriole or a Venule?
| Autor: | Howard Dobson, Theodore P Criswell, Ajay Verma, Ciara Finucane, Matthew MacGregor Sharp, Roxana O. Carare |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Aging Canine brain Cognitive Neuroscience arterioles Lumen (anatomy) intramural cell lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine Smooth muscle Arteriole medicine.artery transmission electron microscopy morphology medicine lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research Venule Chemistry Anatomy Lumen Diameter 030104 developmental biology venules cardiovascular system Area ratio Wall thickness 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Aging Neuroscience, Vol 11 (2019) Frontiers in Aging Neuroscience |
| ISSN: | 1663-4365 |
| Popis: | There are very few reliable methods in the literature to discern with certainty between cerebral arterioles and venules. Smooth muscle cells (SMC) and pericytes are present in both arterioles and venules, so immunocytochemistry for markers specific to intramural cells (IMC) is unreliable. This study employed transmission electron microscopy (TEM) and a canine brain to produce robust criteria for the correct identification of cerebral arterioles and venules based on lumen:vessel wall area, tested against the less accurate lumen diameter:vessel wall thickness. We first used morphology of IMC to identify two distinct groups of vessels; group 1 with morphology akin to venules and group 2 with morphology akin to arterioles. We then quantitatively assessed these vessels for lumen:vessel wall area ratio and lumen diameter:wall thickness ratio. After assessing 112 vessels, we show two distinct groups of vessels that can be separated using lumen:vessel wall area (group 1, 1.89 -10.96 vs. group 2, 0.27-1.57; p < 0.001) but not using lumen diameter:vessel wall thickness where a substantial overlap in ranges between groups occurred (group 1, 1.58-22.66 vs. group 2, 1.40-11.63). We, therefore, conclude that lumen:vessel wall area is a more sensitive and preferred method for distinguishing cerebral arterioles from venules. The significance of this study is wide, as cerebral small vessel disease is a key feature of vascular dementia and understanding the pathogenesis relies on correct identification of vessels. |
| Databáze: | OpenAIRE |
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