Genomic content of a novel yeast species Hanseniaspora gamundiae sp. Nov. From fungal stromata (Cyttaria) associated with a unique fermented beverage in Andean Patagonia, Argentina
| Autor: | Neža Čadež, Ricardo Ulloa, Diego Libkind, Nicolás Bellora, Chris Todd Hittinger |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
BEER
Yeast and Fungal Models Hanseniaspora Genome purl.org/becyt/ford/1 [https] NOVEL YEAST DNA Fungal Phylogeny Data Management Genetics Cyttaria hariotii 0303 health sciences Multidisciplinary FERMENTATION biology Phylogenetic tree Patagonian Database and informatics methods Sequence analysis Fungal genetics Eukaryota Phylogenetic Analysis Genomics Bioquímica y Biología Molecular Phylogenetics GENOME Experimental Organism Systems Saccharomyces Cerevisiae Medicine Fagales Genome Fungal CIENCIAS NATURALES Y EXACTAS Research Article Biotechnology Computer and Information Sciences Bioinformatics Science Argentina Research and Analysis Methods Beverages Ciencias Biológicas Saccharomyces 03 medical and health sciences Model Organisms Ascomycota Biología Celular Microbiología Evolutionary Systematics Gene Prediction purl.org/becyt/ford/1.6 [https] Ecosystem DNA sequence analysis Taxonomy 030304 developmental biology Evolutionary Biology 030306 microbiology Organisms Fungi Genetic Variation Biology and Life Sciences Computational Biology Comparative Genomics 15. Life on land Genome Analysis biology.organism_classification Yeast Fermentation Animal Studies Translational elongation Sequence Alignment Cyttaria |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE, Vol 14, Iss 1, p e0210792 (2019) PLoS ONE |
| DOI: | 10.1371/journal.pone.0210792 |
| Popis: | A novel yeast species was isolated from the sugar-rich stromata of Cyttaria hariotii collected from two different Nothofagus tree species in the Andean forests of Patagonia, Argentina. Phylogenetic analyses of the concatenated sequence of the rRNA gene sequences and the protein-coding genes for actin and translational elongation factor-1α indicated that the novel species belongs to the genus Hanseniaspora. De novo genome assembly of the strain CRUB 1928 T yielded a 10.2-Mbp genome assembly predicted to encode 4452 protein-coding genes. The genome sequence data were compared to the genomes of other Hanseniaspora species using three different methods, an alignment-free distance measure, K r , and two model-based estimations of DNA-DNA homology values, of which all provided indicative values to delineate species of Hanseniaspora. Given its potential role in a rare indigenous alcoholic beverage in which yeasts ferment sugars extracted from the stromata of Cytarria sp., we searched for the genes that may suggest adaptation of novel Hanseniaspora species to fermenting communities. The SSU1-like gene encoding a sulfite efflux pump, which, among Hanseniaspora, is present only in close relatives to the new species, was detected and analyzed, suggesting that this gene might be one factor that characterizes this novel species. We also discuss several candidate genes that likely underlie the physiological traits used for traditional taxonomic identification. Based on these results, a novel yeast species with the name Hanseniaspora gamundiae sp. nov. is proposed with CRUB 1928 T (ex-types: ZIM 2545 T = NRRL Y-63793 T = PYCC 7262 T ; MycoBank number MB 824091) as the type strain. Furthermore, we propose the transfer of the Kloeckera species, K. hatyaiensis, K. lindneri and K. taiwanica to the genus Hanseniaspora as Hanseniaspora hatyaiensis comb. nov. (MB 828569), Hanseniaspora lindneri comb. nov. (MB 828566) and Hanseniaspora taiwanica comb. nov. (MB 828567). Fil: Cadez, Neza. University of Ljubljana; Eslovenia Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina. Universidad Nacional del Comahue. Centro Regional Universidad de Bariloche. Departamento de Biología. Laboratorio de Microbiología Aplicada y Biotecnología; Argentina Fil: Ulloa, José Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina Fil: Hittinger, Chris. University of Wisconsin; Estados Unidos Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina |
| Databáze: | OpenAIRE |
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