Disruptions in valine degradation affect seed development and germination in Arabidopsis
| Autor: | Matthew F. Rouhier, Rachel J. Rhee, Jack A. Clayton, Dana L. Oakes, Kerry A Rouhier, Andrew B Gipson, Kyla J. Morton, Szabolcs Simo, Christiana Binkley, Morgan E. Perrett, Erika L. Jensen |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Germination Plant Science 01 natural sciences Article 03 medical and health sciences Amino acid homeostasis Valine Gene Expression Regulation Plant Genetics Arabidopsis thaliana chemistry.chemical_classification biology Catabolism Arabidopsis Proteins Cell Biology biology.organism_classification Plants Genetically Modified Amino acid 030104 developmental biology chemistry Biochemistry Seeds Isoleucine Leucine 010606 plant biology & botany |
| Zdroj: | The Plant journal : for cell and molecular biology. 90(6) |
| ISSN: | 1365-313X |
| Popis: | We have functionally characterized the role of two putative mitochondrial enzymes in valine degradation using insertional mutants. Prior to this study, the relationship between branched-chain amino acid degradation (named for leucine, valine and isoleucine) and seed development was limited to leucine catabolism. Using a reverse genetics approach, we show that disruptions in the mitochondrial valine degradation pathway affect seed development and germination in Arabidopsis thaliana. A null mutant of 3-hydroxyisobutyryl-CoA hydrolase (CHY4, At4g31810) resulted in an embryo lethal phenotype, while a null mutant of methylmalonate semialdehyde dehydrogenase (MMSD, At2g14170) resulted in seeds with wrinkled coats, decreased storage reserves, elevated valine and leucine, and reduced germination rates. These data highlight the unique contributions CHY4 and MMSD make to the overall growth and viability of plants. It also increases our knowledge of the role branched-chain amino acid catabolism plays in seed development and amino acid homeostasis. |
| Databáze: | OpenAIRE |
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