Inhibition of protein kinase C antagonizes in vitro tadpole tail fin regression induced by thyroxine
| Autor: | Douglas W. Petcoff, James E. Platt |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
In Vitro Techniques Cola (plant) Piperazines Rana Endocrinology Salientia Internal medicine 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine medicine Animals Protein kinase C Protein Kinase C Sulfonamides biology Dose-Response Relationship Drug Rana pipiens Metamorphosis Biological biology.organism_classification Isoquinolines In vitro Prolactin Thyroxine GRENOUILLE Animal Science and Zoology |
| Zdroj: | General and comparative endocrinology. 87(2) |
| ISSN: | 0016-6480 |
| Popis: | Tail fin regression can be induced in anuran amphibians with L-thyroxine (T4). This regression can be antagonized with prolactin (PRL). Previous work had suggested that protein kinase C (PKC) was involved in PRL action. To address this issue further, the effect of a potent and selective inhibitor of protein kinase C on in vitro tail fin regression was investigated. T4-induced regression of tail fin pieces from Rana pipiens tadpoles could be antagonized by adding PRL or the PKC inhibitor H-7 to the medium. H-7 inhibited fin regression in a dose-dependent manner, with a half-maximal effective concentration of about 10(-5) M. The H-7 analogue, HA-1004 (which is not a selective inhibitor of PKC), was without effect. These results suggest a possible role for PKC in tail fin regression and may be useful in elucidating the antimetamorphic action of PRL. |
| Databáze: | OpenAIRE |
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