Effect of active immunization with IL-17A on B cell function and infection risk in pristane-induced lupus model
| Autor: | Hanestya Oky Hermawan, Nafisah Nur'aini, Dita Kartika Sari, Mirza Zaka Pratama, Hafishtyawan Maulidyananta Agdana, Dian Hasanah, Kusworini Handono, Keryasta Becik Kawuningan, Handono Kalim |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Methicillin-Resistant Staphylococcus aureus Time Factors Plasma cell Active immunization Lymphocyte Activation Andrology 03 medical and health sciences 0302 clinical medicine Immunogenicity Vaccine Rheumatology Adjuvants Immunologic medicine Animals Lupus Erythematosus Systemic B cell B-Lymphocytes Mice Inbred BALB C Systemic lupus erythematosus biology business.industry Terpenes Interleukin-17 Antibody titer Staphylococcal Infections medicine.disease Antibodies Neutralizing Titer Disease Models Animal 030104 developmental biology medicine.anatomical_structure Antibodies Antinuclear Hemocyanins biology.protein Female Immunization Antibody business Keyhole limpet hemocyanin 030215 immunology |
| Zdroj: | International journal of rheumatic diseases. 21(6) |
| ISSN: | 1756-185X |
| Popis: | PURPOSE The aim of this study was to determine the effect of active immunization of interleukin (IL)-17A to inhibit B cell functions and monitor the risk of infection in a pristane-induced lupus mice model. METHODS Female Balb/c mice were given a single intraperitoneal injection of 0.5 mL pristane. IL-17A was coupled to keyhole limpet hemocyanin (KLH) and given to mice in three different doses: D0 (0 μg/mL), D1 (1 μg/mL), and D2 (10 μg/mL). The vaccine was given three times with 3-week intervals. At day 42, mice were injected intraperitoneally with methicillin-resistant Staphylococcus aureus (MRSA) and monitored for 3 weeks. Plasma cells proliferation, Th17 and plasma cell percentages were measured by flow cytometry; anti-IL-17A antibody titers, IL-17A, and anti-double-stranded DNA (anti-dsDNA) levels were measured by enzyme-linked immunosorbent assay; and MRSA colonization was measured by bacterial counter. RESULTS Anti-IL-17A antibody titers were significantly higher in D2 compared to D0 (P = 0.012). Serum IL-17A levels were also significantly lower in D2 compared to D0 (P = 0.000) while Th17 percentages were not significantly different between groups. D2 was also had significantly lower anti-dsDNA (P = 0.021), lower plasma cell percentages (P = 0.000) and lower B cell proliferation rate (P = 0.001) compared to D0. Analysis for the risk of infection also revealed that D2 did not increase the risk of infection compared to D0 (P = 0.504). CONCLUSION Active immunization with IL-17A coupled to KLH was able to induce a high titer of neutralizing antibodies against IL-17A and inhibit B cell functions without increasing the risk of infection in a pristane-induced lupus mice model. |
| Databáze: | OpenAIRE |
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