Cytokine gene expression in skin of susceptible guinea‐pig infected withTreponema pallidum
| Autor: | K Wicher, A M Scarozza, Victoria Wicher, Arlene I. Ramsingh |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Necrosis Guinea Pigs Immunology Gene Expression Virulence Peripheral blood mononuclear cell Microbiology Guinea pig Immune system Transforming Growth Factor beta Eosinophilia Gene expression medicine Animals Immunology and Allergy Syphilis Treponema pallidum Pathogen Skin Treponema biology Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha biology.organism_classification Interleukin-12 Interleukin-10 Cytokines Interleukin-2 Rabbits medicine.symptom Research Article Interleukin-1 |
| Zdroj: | Immunology. 95:242-247 |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1046/j.1365-2567.1998.00596.x |
| Popis: | Using a semi-quantitative multiplex reverse transcription-polymerase chain reaction assay, we examined cytokine mRNA expression for interleukin-1alpha (IL-1alpha), IL-2, IL-10, IL-12p40, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) in skin samples obtained from C4-deficient (C4D) guinea-pigs inoculated intradermally with virulent Treponema pallidum (VTP). Controls included unmanipulated animals, guinea-pigs injected with T. pallidum-free rabbit inflammatory testicular fluid (ITF) alone, or mixed with heat-killed organisms (HKTP). The expression of IL-1alpha, IL-12p40, and TNF-alpha mRNA [T helper type 1 (Th1)] remained within the normal range in both infected and control animals throughout the experimental period. However, a significant increase (P |
| Databáze: | OpenAIRE |
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